产品说明
一般描述
The Human Ubiquitin+1 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human Ubiquitin+1 in serum, plasma, cell culture supernatants and urine.
The RPS27A (ribosomal protein S27a) gene is mapped to human chromosome 2p16.1. The protein complex encoded contains ubiquitin at the N-terminus and a C-terminal ribosomal protein S27a.
免疫原
Recombinant Human Ubiquitin
应用
请参考Protocol了解详情。
生化/生理作用
The ribosomal protein S27a (Rps27a) possesses two main functions, it is responsible for ribosome formation and takes part in post translational modification of proteins. DNA damage elevates the RPS27A gene expression. The RPS27A gene is upregulated in chronic myelogenous leukemia and colorectal cancer. It is known to stimulate cell cycle by inducing cellular checkpoints through p53 and cell proliferation and prevent apoptosis in leukemia cells.
Ribosomal protein S27a (RPS27a), also known as ubiquitin carboxyl extension protein 1 (UBCEP-1), is involved in ribosome biogenesis and post-translational modifications of proteins. The encoded protein enhances cell proliferation, controls cell cycle progression and inhibits apoptosis of leukemia cells. Overexpression of RPS27A results in advanced-phase chronic myeloid leukemia (CML) and acute leukemia (AL). Hence drugs targeting RPS27a combining with tyrosine kinase inhibitor (TKI) might be a potential treatment for TKI resistant CML patients. Elevated expression of UBCEP-1, which is a component of ubiquitin pathway, promotes prostate carcinoma development, via increasing the degradation of proteins involved in growth inhibition or apoptosis. RPS27a might act as a unique stress sensor in cells, which results in increased p53 activity leading to inhibition of cell cycle progression.
其他说明
A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.
产品性质
| species reactivity | human |
| 包装 | kit of 96 wells (12 strips x 8 wells) |
| technique(s) | ELISA: suitable capture ELISA: suitable |
| input | sample type serum sample type urine sample type plasma sample type cell culture supernatant(s) |
| assay range | inter-assay cv: <12% intra-assay cv: <10% sensitivity: 30 pg/mL standard curve range: 27.43-20000 pg/mL |
| detection method | colorimetric |
| 运输 | wet ice |
| 储存温度 | −20℃ |
| Gene Information | human ... RPS27A(6233) |
组份列表
组份可单独销售
| 货号 | 组份 |
| RABELADB | ELISA 5X Assay/Sample Diluent Buffer B (Item E1) |
| RABELADC | ELISA 1X Assay/Sample Diluent Buffer C (Item L) |
| RABSTOP3 | ELISA Stop Solution (Item I) |
| RABTMB3 | ELISA Colorimetric TMB Reagent (HRP Substrate, Item H) |
| RABWASH4 | 20X Wash Buffer (Item B) |
安全信息
| 象形图 |  |
| 警示用语： | Warning |
| 危险声明 | H290 |
| 预防措施声明 | P234 - P390 |
| 危险分类 | Met. Corr. 1 |
| 储存分类代码 | 8A - Combustible, corrosive hazardous materials |
| WGK | WGK 3 |
