产品说明
一般描述
Human GSK3beta GenBank Accession No. NM_002093), full length with N-terminal GST-tag, MW=73 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
Glycogen Synthase Kinase 3β (GSK3β) is one of the two isoforms of GSK3 enzyme, both of which are encoded by different genes. This protein is predominantly cytoplasmic, but is also found to be localized to nucleus and mitochondria. It is a protein-serine kinase. This protein contains a protein kinase domain in its core, and is composed of 482 amino acids with a molecular weight of 46,712Da. It shows a wide range of tissue expression with the highest expression in brain.
应用
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
生化/生理作用
Glycogen Synthase Kinase 3β (GSK3β) is responsible for rapid cyclin D1 turnover by phosphorylating it on Thr-286. It functions as a negative regulator of Wnt signaling during embryonic development and cell proliferation in adult tissues. This protein phosphorylates and regulates multiple proteins such as glycogen synthase, insulin receptor substrate-1, axin and APC (adenomatous polyposis coli). This protein is also shown to interact with multiple protein which are linked with Alzheimer′s disease (AD).
基本信息
| NACRES | NA.32 |
产品性质
| 生物来源 | human |
| 重组 | expressed in baculovirus infected Sf9 cells |
| 测定 | ≥80% (SDS-PAGE) |
| 形式 | aqueous solution |
| specific activity | ≥500 pmol/min-μg |
| 分子量 | 73 kDa |
| 包装 | pkg of 10 μg |
| technique(s) | activity assay: suitable inhibition assay: suitable |
| NCBI登记号 | NM_002093 |
| UniProt登记号 | P49841 |
| 运输 | dry ice |
| 储存温度 | −70℃ |
| Gene Information | human ... GSK3B(2932) |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | WGK 1 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
