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Glycolysis Assay Kit

品牌
Sigma-Aldrich
货号
MAK439
规格纯度
Sufficient for 100 colorimetric tests
参考价格
4523.37 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

Glycolysis is one of the major metabolic pathways cells undergo to produce energy and results in the production of pyruvate. One of the eventual end products of this process is lactate dehydrogenase-mediated conversion of pyruvate to L-lactate via lactic acid fermentation. This enzymatic mechanism allows L-lactate to serve as an indicator of glycolysis.

特点和优势

  • Linear detection range up to 10 mM L-lactate in 96-well plate assay.
  • Convenient. The procedure is performed at room temperature and involves adding a single working reagent.
  • Compatible with high-throughput handling systems.

适用性

The kit is suitable for the direct determination of L-Lactate produced by glycolysis in cell media samples and for the screening of glycolysis inhibitors.

原理

The Glycolysis Assay Kit is based on measuring the production of L-Lactate from glycolysis in cells. L-Lactate that is secreted into the cell media is quantified using a coupled reaction involving the lactate dehydrogenase catalyzed oxidation of L-lactate that generates pyruvate and NADH, which subsequently reduces a formazan dye. The intensity of the reduced dye, measured at 565 nm, is directly proportional to the L-lactate concentration in the sample, which in turn is directly proportional to the glycolytic rate of the cells.

产品性质

质量水平100
detection methodcolorimetric
相关疾病diabetes; cancer; endocrinological disorders
储存温度−20℃

安全信息

储存分类代码10 - Combustible liquids
WGKWGK 2

Sigma-Aldrich

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