Glutathione GSH/GSSG Assay Kit

产品说明

一般描述

Glutathione, a tripeptide of glycine, glutamic acid, and cysteine, is one of the key antioxidants involved in protecting cells from damage by reactive oxygen species. Glutathione (GSH) reduces disulfide bonds in cytoplasmic proteins to cysteines, in which it is converted to its oxidized form GSSG.

特点和优势

• Linear detection range 0.01-3 μM GSH equivalents with a detection limit of 10 nM GSH equivalents.

适用性

The kit is suitable for the quantitative determination of reduced and oxidized glutathione (GSH/GSSG) in whole blood, plasma, serum, urine, tissue and cell extracts.

原理

The Glutathione GSH/GSSG Assay Kit is designed to accurately measure total, reduced and oxidized glutathione in biological samples using an enzymatic method that utilizes Ellman′s Reagent (DTNB) and glutathione reductase (GR). DTNB reacts with reduced glutathione to form a yellow product. The rate of change in the optical density, measured at A₄₁₂ nm, is directly proportional to the glutathione concentration in the sample. This kit can also be used to measure oxidized (GSSG) by using a specific protocol which first scavenges all GSH with 1-methyl-2-vinylpyridinium triflate.

产品性质

安全信息