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Glucose-6-Phosphate Dehydrogenase Inhibitor Screening Kit

品牌
Sigma-Aldrich
货号
MAK452
规格纯度
Sufficient for 100 colorimetric tests
参考价格
5056.96 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

Glucose-6-Phosphate Dehydrogenase (G6PDH) is a cytosolic enzyme in the pentose phosphate pathway which supplies reducing energy to cells by maintaining the level of the co-enzyme nicotinamide adenine dinucleotide phosphate (NADPH). G6PDH reduces nicotinamide adenine dinucleotide phosphate (NADP) to NADPH while oxidizing glucose-6-phosphate (G6P). Humans with a genetic deficiency of G6PDH are predisposed to non-immune hemolytic anemia. Recently, studies have found G6PDH plays a critical role in survival, proliferation, and metastasis of cancer cells. Therefore, inhibitors of the enzyme are attractive candidates for new cancer therapeutics.

特点和优势

  • Compatible with high-throughput handling systems.

适用性

The kit is suitable for high throughput inhibitor screening and evaluation of glucose-6-phosphate dehydrogenase inhibitors.

原理

The Glucose-6-Phosphate Dehydrogenase Inhibitor Screening Kit is based on the reduction of the tetrazolium salt MTT in a NADPH-coupled enzymatic reaction to a reduced form of MTT which exhibits an absorption maximum at A565 nm. The increase in absorbance at A565 nm is proportional to the enzyme activity. The percent inhibition of a test compound can be determined by comparing the activity of G6PDH treated with a test compound to the activity of untreated G6PDH.

产品性质

质量水平100
detection methodcolorimetric
相关疾病hematological disorder, anemia; cancer
储存温度−20℃

安全信息

象形图GHS08
警示用语:Warning
危险声明H341
预防措施声明P201 - P202 - P280 - P308 + P313 - P405 - P501
危险分类Muta. 2
储存分类代码10 - Combustible liquids
WGKWGK 3

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