产品说明
一般描述
Glucose-6-Phosphate Dehydrogenase (G6PDH) is a cytosolic enzyme in the pentose phosphate pathway which supplies reducing energy to cells by maintaining the level of the co-enzyme nicotinamide adenine dinucleotide phosphate (NADPH). G6PDH reduces nicotinamide adenine dinucleotide phosphate (NADP) to NADPH while oxidizing glucose-6-phosphate (G6P). Humans with a genetic deficiency of G6PDH are predisposed to non-immune hemolytic anemia.
特点和优势
- Linear detection range (20 μL sample): 0.2 to 100 U/L for 15 minute reaction.
- Compatible with high-throughput handling systems.
适用性
The kit is suitable for the quantitative determination of Glucose-6-Phosphate concentration and evaluation of drug effects on its metabolism in plasma, serum, tissue and culture media, etc.
原理
The non-radioactive, colorimetric Glucose-6-Phosphate Dehydrogenase Assay Kit is based on the reduction of tetrazolium salt MTT in a NADPH-coupled enzymatic reaction to a reduced form of MTT which exhibits an absorption maximum at A565 nm. The increase in absorbance at A565 nm is proportional to the enzyme activity.
产品性质
| 质量水平 | 100 |
| detection method | colorimetric |
| 相关疾病 | hematological disorder, anemia |
| 储存温度 | −20℃ |
安全信息
| 象形图 |  |
| 警示用语： | Warning |
| 危险声明 | H341 |
| 预防措施声明 | P201 - P202 - P280 - P308 + P313 - P405 - P501 |
| 危险分类 | Muta. 2 |
| 储存分类代码 | 10 - Combustible liquids |
| WGK | WGK 3 |
