产品说明
一般描述
Glial fibrillary acidic protein (UniProt F1RR02) is encoded by the GFAP gene (Gene ID 396562) in porcine. GFAP is a type III intermediate filament (IF) thought to help maintain astrocyte mechanical strength and is closely related to vimentin, desmin, and peripherin, which are involved in the structure and function of cellular cytoskeleton. GFAP is expressed in astrocytes in the central nervous system (CNS), where it is involved in many important CNS processes, including cell communication and the blood brain barrier (BBC) function. GFAP contains the characteristic tripartite domain structure among IF members, a central α-helical rod domain (a.a. 73-377) flanked by a non-α-helical N-terminal head (a.a. 1-72) and a C-terminal tail (a.a. 378-432) domain. The head and tail domains are most structurally variable among IF proteins and their contributions to filament assembly vary depending on the IF proteins. For GFAP, the tail domain is reported to be important for filament assembly. The tail domain R416W mutation is linked to the neurological disorder Alexander disease (AxD).
Purified porcine Glial Fibrillary Acidic Protein (GFAP) purified from spinal cord.
外形
Liquid. Buffer = PBS.
储存及稳定性
Maintain at -20°C to -70°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.
During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 μL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container′s cap.
分析说明
a 50 kDa single band by SDS-PAGE.
法律信息
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| eCl@ss | 32160405 |
| NACRES | NA.41 |
产品性质
| 生物来源 | Porcine |
| 质量水平 | 100 |
| 测定 | >99% (pure GFAP) |
| 分子量 | calculated mol wt 49.44 kDa (428 a.a. spliced isoform; NP_001231326) calculated mol wt 53.63 kDa (468 a.a. spliced isoform; XP_005668766) |
| manufacturer/tradename | Chemicon® |
| 浓度 | 1 mg/mL |
| technique(s) | ELISA: suitable radioimmunoassay: suitable western blot: suitable |
| NCBI登记号 | NM_001244397 |
| UniProt登记号 | F1RR02 |
| 运输 | dry ice |
| Gene Information | pig ... GFAP(396562) |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | WGK 2 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
