产品说明
一般描述
Human G9a (euchromatic histone-lysine N-methyltransferase 2) with N-terminal GST tag, MW = 74.6 kDa, expressed in an E. coli expression system. G9a is a member of SET (Su(var)3-9, enhancer-of-zeste and trithorax) domain containing, Su(var)3-9 family of proteins. This domain is evolutionary conserved.
应用
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
生化/生理作用
The encoded protein G9a (euchromatic histone-lysine N-methyltransferase 2) is a methyltransferase that methylates lysine residues of histone H3. Overexpression of G9a is observed in cancer. It is known to participate in cancer cell migration, invasion and metastasis. G9a is an epigenetic regulator and can inhibit the action of tumor suppressor genes, thereby, contributes to carcinogenesis. Thus, G9a is considered to be an oncogenic and metastatic factor. It also affects DNA damage repair mechanism, which might make tumor cells resistant to radiation and chemotherapy. G9a serves as a target for cancer therapy including breast cancer.
单位定义
One unit is defined as the amount of enzyme required to methylate 1 pmol of substrate/min at 37℃.
外形
Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 30% glycerol and 3 mM DTT.
制备说明
Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70℃. Note: Enzyme is very sensitive to freeze/thaw cycles.
基本信息
| NACRES | NA.32 |
产品性质
| 生物来源 | human |
| 重组 | expressed in E. coli |
| 测定 | ≥70% (SDS-PAGE) |
| 形式 | aqueous solution |
| 分子量 | 74.6 kDa |
| 包装 | pkg of 50 μg |
| 浓度 | >0.02 mg/mL |
| NCBI登记号 | NM_006709 |
| UniProt登记号 | Q96KQ7 |
| 运输 | dry ice |
| 储存温度 | −70℃ |
| Gene Information | human ... EHMT2(10919) |
安全信息
| 储存分类代码 | 10 - Combustible liquids |
| WGK | WGK 1 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
