产品说明
一般描述
A common problem associated with the routine culture of human pluripotent stem cells, including ES and iPS cells, is the unwanted spontaneous differentiation of cells. Without manual intervention, differentiated cells proliferate quickly and eventually diminish the overall quality of the pluripotent stem cell culture. Manual removal of differentiated cells is laborious and time consuming, making it difficult to process multiple hES/iPS cell lines simultaneously. Moreover, the risk for contamination is present each time the culture is subjected to this clean-up process.
EZ-LiFT
EZ-LiFT does not suffer from the drawbacks of other enzyme-based dissociation reagents such as Dispase, Collagenase IV and Accutase. The disadvantages of enzyme-based reagents are that they still require manual removal of differentiated cells, suffer from lot-to-lot variability and may carry the risk for karyotypic abnormalities.
Features and Benefits
• Enzyme-free and chemically defined formulation: Produces consistent results.
• Enriches for pluripotent stem cell populations: No manual clean-up required.
• Rescues highly differentiated hES/iPS culture: Saves precious samples and time.
• Gentle on cells with high cell survival rates: ROCKi is optional, but not required.
应用
Research Category
Cell Culture
Stem Cell Research
质量
pH: 7.2-7.4
Sterility Tested: Pass
Osmolarity: 340-370 mOsm
储存及稳定性
EZ-LiFT Stem Cell Passaging Reagent can be stored at room temperature for 1 year without loss of activity.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
eCl@ss | 32160801 |
产品性质
形式 | liquid |
technique(s) | cell culture | stem cell: suitable |
安全信息
储存分类代码 | 12 - Non Combustible Liquids |
WGK | WGK 1 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |