产品说明
一般描述
Introduction:
Cell adhesion plays a major role in cellular communication and regulation, and is of fundamental importance in the development and maintenance of tissues. Scientists are continually examining the adhesion, migration, and invasion of many diverse cell types on various extracellular matrix (ECM) component proteins. The CHEMICON® ECM Cell Adhesion Array Kit (Fluorometric) provides an ECM Array microtiter plate with a homogenous fluorescence detection format, allowing for large-scale screening and quantitative comparison of multiple samples/cell lines. The kit is designed as a rapid (1-2 hours), cost effective, efficient method for characterization of cell adhesion.
In addition, Chemicon continues to provide numerous migration, invasion, and adhesion products including:
· Alpha Integrin-Mediated Cell Adhesion Array Kit (Colorimetric) (ECM530)
· Beta Integrin-Mediated Cell Adhesion Array Kit (Colorimetric) (ECM531)
· Alpha/Beta Integrin-Mediated Cell Adhesion Array Combo Kit (Colorimetric) (ECM532)
· Alpha Integrin-Mediated Cell Adhesion Array Kit (Fluorometric) (ECM533)
· Beta Integrin-Mediated Cell Adhesion Array Kit (Fluorometric) (ECM534)
· Alpha/Beta Integrin-Mediated Cell Adhesion Array Combo Kit (Fluorometric) (ECM535)
· ECM Cell Adhesion Array Kit (Colorimetric) (ECM540)
· QCM
· QCM
· QCM
· QCM
· QCM
· 24-well Insert Cell Migration and Invasion assay systems
· CytoMatrix
· QuantiMatrix
Test Principle:
The CHEMICON
Additional information about the ECM components provided in the kit:
包装
1 plate in 96 wells
96 wells
应用
Research Category
Cell Structure
The ECM Cell Adhesion Array kit is useful for assessing specific cell surface integrins, monitoring in vitro cell differentiation, or screening potential cell adhesion promoters/inhibitors.
For Research Use Only. Not for use in diagnostic procedures.
The ECM Cell Adhesion Array kit is useful for assessing specific cell surface integrins, monitoring in vitro cell differentiation, or screening potential cell adhesion promoters/inhibitors.
组分
ECM Array Plate: (PN: 90652) One 96-well plate with 12 strips. Each 8-well strip consists of 7 different human, ECM protein-coated wells (Collagen I, Collagen II, Collagen IV, Fibronectin, Laminin, Tenascin, Vitronectin) and one BSA-coated well (negative control). See the plate layout on data sheet.
4X Cell Lysis Buffer: (Part No. 90130) One bottle - 16 mL.
CyQuant GR™ Dye: (Part No. 90132) One vial - 75 μL.
Assay Buffer: (Part No. 90601) One bottle - 100 mL.
储存及稳定性
The ECM Array Plate can be stored at 2° to 8°C in the foil pouch up to its expiration date. Unused strips may be placed back in the pouch for storage. Ensure that the desiccant remains in the pouch, and that the pouch is securely closed. Keep the remaining kit components at 2° to 8°C.
Precautions:
No data is available on the biological toxicity of CyQuant GRâ dye. This reagent binds to nucleic acids, and as such, should be treated as a potential mutagen which may cause cancer and heritable genetic damage. Handle with caution. The DMSO stock solution should be handled with special caution as DMSO can facilitate the entry of organic molecules into tissues.
法律信息
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
GR is a trademark of sigma-aldrich Co. LLC
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
eCl@ss | 32161000 |
产品性质
质量水平 | 100 |
species reactivity | human |
manufacturer/tradename | Chemicon® |
technique(s) | cell based assay: suitable |
detection method | fluorometric |
运输 | wet ice |
安全信息
储存分类代码 | 10 - Combustible liquids |