产品说明
应用
Specificity
Fluorescence detection reagents are often used with FITC, Cy®3,Texas Red® or Cy®5 filter. The filter chosen is dependent on the color of the Multicolor Probemaker kit selected.
Application Note
Primary antibodies are needed. Test your primary antibodies (IgG-class, mono- or polyclonal) in a standard immunofluorescence (IF), immunohistochemistry (IHC), or immunocytochemistry (ICC) assay to determine the optimal fixation, blocking, and titer conditions. Duolink® in situ reagents are suitable for use on fixed cells, cytospin cells, cells grown on slide, formalin-fixed, paraffin embedded (FFPE), or tissue (fresh or frozen). No minimum number of cells is required.
Let us do the work for you, learn more about our Custom Service Program to accelerate your Duolink® projects
View full Duolink® product list
特点和优势
Multicolor Duolink® proximity ligation assay (PLA®) is an application of the Duolink® technology that allows for endogenous, simultaneous detection of up to 4 protein interactions, post-translational modifications, or protein expression levels (including low abundant protein) in fixed cells and tissue samples. Follow the Multicolor Duolink PLA protocol to use this product.
- Visualize and detect multiple proteins events in one experiment.
- No overexpression or genetic manipulation required
- High specificity (fewer false positives)
- Detection of low abundant proteins due to rolling circle amplification
- Relative quantification possible
- No special equipment needed
- Faster and more simple than FRET
- Increased accuracy compared to co-IP
- Publication-ready results
Components
Duolink® PLA Multicolor Reagent Pack contains all the necessary reagents to perform the amplification and detection of bound custom PLA probes generated using Duolink® PLA Multicolor Probemaker Kits. Sufficient components are provided for the indicated number of reactions (30 or 100 RXN), based on 40 mL of the total reaction mixture covering 1 cm2.
- This product is comprised of the following:
- 5X Multicolor PLA LIgation Buffer DUO86002
- Ligase DUO82027 or DUO82025
- 5X Amplification Buffer DUO82050
- Polymerase DUO82028 or DUO82026
- 5X Multicolor PLA Detection Buffer DUO86001
- 1X Blocking Buffer DUO82007
- 1X Probemaker PLA Probe Diluent DUO82036
See datasheet for more information.
To perform a Multicolor Duolink® PLA experiment you will need primary antibodies (PLA, IHC, ICC or IF validated) that recognize target epitopes. You will also need at least two pair of the resulting Duolink® PLA Multicolor Probemaker-generated probes (Red A/Red B, Green C/Green D, Orange F/Orange G, and/or FarRed H/FarRed I).The reagents in the Duolink® PLA Multicolor Reagent Pack are required to perform the amplification and detection of bound Multicolor PLA probes. Recommended additional reagents include Duolink® Wash Buffers and Mounting Medium.
法律信息
Cy is a registered trademark of Cytiva
Duolink is a registered trademark of sigma-aldrich Co. LLC
PLA is a registered trademark of sigma-aldrich Co. LLC
Texas Red is a registered trademark of Life Technologies
产品性质
| 产品线 | Duolink® |
| technique(s) | immunofluorescence: suitable multiplexing: suitable proximity ligation assay: suitable |
| 适用性 | suitable for fluorescence |
| 运输 | dry ice |
| 储存温度 | −20℃ |
