产品说明
一般描述
Protein tyrosine phosphatase (density-enhanced phosphatase-1, PTP-eta, CD148), Catalytic domain, (amino acids 1024-1306) (Gene bank accession no. NM_U10886) with N-terminal GST tag, MW=58.9 kDa, expressed in an E. coli expression system.
应用
Useful for the study of enzyme kinetics, regulation, and to dephosphorylate target substrates.
单位定义
One unit will hydrolyze 1 nmol p-nitrophenyl phosphate per minute at pH 7.4 and 30℃.
外形
Formulated in 25 mM Tris-HCl, pH 8.0, 75 mM NaCl, 0.05% Tween-20, 50% glycerol, 2 mM EDTA, 1 mM DTT, and 10 mM glutathione.
制备说明
Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70℃. Note: Enzyme is very sensitive to freeze/thaw cycles.
基本信息
| NACRES | NA.32 |
产品性质
| 生物来源 | human |
| 重组 | expressed in E. coli |
| 测定 | ≥90% (SDS-PAGE) |
| 形式 | aqueous solution |
| 效能 | ≥0.114 units/mg |
| 分子量 | 58.9 kDa |
| 包装 | pkg of 20 μg |
| 浓度 | >0.02 mg/mL |
| NCBI登记号 | NM_U10886 |
| UniProt登记号 | Q12913 |
| 运输 | dry ice |
| 储存温度 | −70℃ |
| Gene Information | human ... PTPRJ(5795) |
