产品说明
一般描述
ColorWheel® technology is a novel and proprietary method of creating your own antibody and dye combinations for use in flow cytometry. By incubating any ColorWheel® antibody with any ColorWheel® dye, researchers can quickly and simply produce primary conjugated antibodies for use in single color or multicolor flow cytometry analysis. Each ColorWheel® antibody and dye is lyophilized for long long-term storage, allowing for simplicity and flexibility without compromise. ColorWheel® technology requires both ColorWheel® antibody and ColorWheel® dye for Flow Cytometry application.
iFluor 633 succinimidyl ester is bright, stable far-red fluorescent dye that has spectral properties comparable to Alexa Fluor® 633. It exhibits an excitation max at 640 nm and an emission max at 654 nm with an extinction coefficient of 250,000 cm-1 M-1. iFluor 633 can be used for covalent labeling of antibodies and detection by flow cytometry. It exhibits good stability, selectivity, and reactivity with protein amine groups.
应用
Flow Cytometry: Excitation: 640 nm, Emission: 654 nm.
相容性
Requires ColorWheel® Antibody (Sold Separately)
外形
CWDS-IF633-25UL Set Components
CWD-IF633-25UL - ColorWheel® Antibody-Ready iFluor 633 Dye
Lyophilized from PBS containing D-Mannitol and Sucrose, normal appearance is a dried pellet. Reconstituted solution is stable and functional as assessed by functional testing. Contains no biocide or preservatives, such as azide.
CWD-FINISH-25UL - ColorWheel® Finishing Buffer
Lyophilized from PBS. Contains no biocide or preservatives, such as azide.
重悬
Reconstitute each vial by adding 25 μL PBS. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
储存及稳定性
Recommend storage of lyophilized product at 2-8℃. Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial. Reconstitute each vial by adding 25 μL of PBS. Protect from light.
法律信息
ALEXA FLUOR is a registered trademark of Life Technologies
ColorWheel is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
产品性质
| 质量水平 | 200 |
| 产品线 | ColorWheel® |
| 形式 | lyophilized |
| technique(s) | flow cytometry: suitable |
| 荧光 | λex 640 nm; λem 654 nm |
| 相容性 | for use with ColorWheel® Dye-ready Antibodies (Required, sold separately) |
| 运输 | ambient |
| 储存温度 | 2-8℃ |
