产品说明
一般描述
Hermansson described the use of natural proteins immobilized onto a silica support for chiral separations in 1983 (1). Proteins contain a large number of chiral centers of one configuration, and many other sites that contribute to the general retention process. Used primarily for the separation enantiomers of basic compounds, CHIRALPAK CBH phase has cellobiohydrolase as the chiral selector immobilized on spherical 5 μm silica particles. Used in reversed-phase mode, retention and enantioselectivity is regulated by changes of pH, buffer concentration and organic modifier. The mobile phases are mixtures of phosphate or acetate buffers and organic solvents such as 2-propanol or acetonitrile. The column is preferably used for the separation of enantiomers of basic drugs, particularly compounds containing one or more nitrogen atoms along with one or more hydrogen-bonding groups (alcohol, phenol, carbonyl, amide, ether, ester, etc.).
Features:
- Direct reversed phase resolution of chiral molecules
- Stable with a variety of organic modifiers
- Available in analytical and semi-preparative sizes
(1) Hermansson, J. Direct liquid chromatographic resolution of racemic drugs using a1-acid glycoprotein as the chiral stationary phase. J. Chromatogr. A, 1983, 269, 71-80.
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法律信息
CHIRALPAK is a registered trademark of Daicel Corp.
基本信息
| eCl@ss | 32110501 |
| NACRES | SB.52 |
产品性质
| 物料 | stainless steel column |
| 质量水平 | 100 |
| 产品线 | CHIRALPAK® |
| 包装 | pkg of 1 ea |
| manufacturer/tradename | CHIRALPAK® |
| 参数 | 137 bar pressure (2000 psi) 20-30 ℃ temperature 40 ℃ max. temp. |
| technique(s) | HPLC: suitable |
| 长度 × 内径 | 10 cm × 4 mm |
| 基质 | fully porous particle |
| 基质活性基团 | cellobiohydrolase phase |
| 粒径 | 5 μm |
| operating pH | 2-8 |
| 分离技术 | chiral |
