产品说明
一般描述
Caspases are members of the aspartate-specific cysteinyl protease family that play a central role in apoptosis. Apoptosis is involved in a variety of physiological and pathological events, ranging from normal fetal development to diseases such as cancer, organ failure, and neurodegenerative diseases. Caspase-3 is key biomarker in the assessment of apoptosis and in understanding mechanism of apoptosis induction.
特点和优势
- Compatible with high-throughput handling systems.
适用性
The kit is suitable for the quantitative determination of the apoptosis target caspase-3 activity and high throughput screening for apoptosis inducers and inhibitors in cell and tissue lysate.
原理
The Caspase-3 Assay Kit provides a convenient means to measure caspase-3 activity in biological samples. In the assay, a specific substrate (N-Ac-DEVD-AFC) is cleaved by active caspase-3, forming a highly fluorescent product. The fluorescence intensity (λEx = 400 nm/λEm = 490 nm) is proportional to the caspase-3 activity.
产品性质
| 质量水平 | 100 |
| detection method | fluorometric |
| 相关疾病 | neurological disorders; cancer |
| 储存温度 | −20℃ |
安全信息
| 象形图 |  |
| 警示用语： | Danger |
| 危险声明 | H315 - H318 |
| 预防措施声明 | P264 - P280 - P302 + P352 - P305 + P351 + P338 - P332 + P313 - P362 + P364 |
| 危险分类 | Eye Dam. 1 - Skin Irrit. 2 |
| 储存分类代码 | 10 - Combustible liquids |
| WGK | WGK 3 |
| 闪点(F) | 188.6 °F |
| 闪点(C) | 87 ℃ |
