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BioTracker TP-HOCL1 Live Cell Dye

品牌
Sigma-Aldrich
货号
SCT043
规格纯度
Live cell imaging dye for hypochlorus acid (HOCL) used to detect phagocytosis, inflammation and cellular apoptosis.
参考价格
3432.94 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

Hypochlorous acid (HOCl) is a highly potent reactive oxygen species (ROS) and helps eliminate pathogens in the innate immune system. Mounting evidence indicates that intracellular HOCl plays additional important roles in regulating inflammation and cellular apoptosis. Subcellular detection of HOCL is currently limited due to low concentration, strong oxidization, and short lifespan of the analyte.

The BioTracker TP-HOCL1 dye is a live cell two-photon green fluorescent “turn-on” imaging probe for HOCL. The probe exhibit fast response times, good selectivity, and high sensitivity towards hypochlorous acid in living cells. Acedan was chosen as the fluorescence reporting group due to its excellent photophysical properties resulting from the typical “push−pull” (amineketone) structure. 2- mercaptoethanol and 1, 2-ethanedithiol were employed to protect the ketone of acedan in the design of HOCl probes. Reaction of the probe with HOCl, which deprotects the oxathiolane/mercaptal group to reveal the ketone, would lead to fluorescence enhancement

Spectral Properties
Absorbance: 375nm
Emission: 500nm

References
Chang YT et al. Development of Targetable Two-Photon Fluorescent Probes to Image Hypochlorous Acid in Mitochondria and Lysosome in Live Cell and Inflamed Mouse. Model. J Am Chem Soc. 2015 May 13;137(18):5930-8.

应用

Research Sub Category
Live Cell Dye
Research Category
Cell Imaging
Live cell fluorescent imaging

质量

Purity: ≥ 98% confirmed by HNMR, LC-MS and HPLC and elemental analysis
Molar Mass: 258.36 g/mol

外形

Lyophilized

储存及稳定性

Store BioTracker TP-HOCL1 Live Cell Dye at -20°C, desiccate and protect from light
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

产品性质

测定≥98% (H-NMR)
≥98% (HPLC)
≥98% (LC/MS)
≥98% (elemental analysis)
technique(s)cell based assay: suitable
detection methodfluorometric
运输ambient

安全信息

储存分类代码11 - Combustible Solids
WGKWGK 3
闪点(F)Not applicable
闪点(C)Not applicable

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