产品说明
应用
Apyrase is used to hydrolyze nucleoside triphosphates and diphosphates. For hydrolysis of organic di and triphosphates, the optimal pH is 6, and for inorganic substrates, the optimal pH is 5.1. Apyrase, from Sigma, has been used in inhibition studies of platelet-aggregation.
在多种马铃薯普通栽培种中至少发现了两种同工酶：一种具有高 ATP 酶/ADP 酶比率(约 10)，另一种该比率较低(约 1)。
反应：ATP → ADP+Pi → AMP+2Pi。
生化/生理作用
Apyrase is found in all eukaryotes and some prokaryotes. Apyrase, from potato, has a crucial role in regulating growth and development. Apyrase is involved in the inactivation of synaptic ATP as a neurotransmitter following nerve stimulation and in the inhibition of ADP induced platelet aggregation to prevent thrombosis. Divalent metal ions are required for activity and best activity is observed with calcium ion at 5 mM.
单位定义
One unit will liberate 1.0 μmole of inorganic phosphate from ATP or ADP per min at pH 6.5 at 30 ℃.
外形
Partially purified, lyophilized powder containing potassium succinate buffer salts.
制备说明
按 ATP 酶单位销售。
其他说明
该等级具有低 ATP 酶/ADP 酶比率。
基本信息
| 酶学委员会(EC)编号 | 3.6.1.5 ( BRENDA | IUBMB ) |
| EC 号 | 232-569-8 |
| MDL编号 | MFCD00130542 |
| NACRES | NA.32 |
产品性质
| 质量水平 | 400 |
| 类型 | Grade VII |
| 形式 | lyophilized powder |
| ATPase activity | ≥200 units/mg protein |
| secondary activity | ≥50 % of base activity ADPase |
| 组成 | Protein, 25-60% |
| 溶解性 | H2O: soluble 1.0 mg/mL |
| application(s) | diagnostic assay manufacturing |
| 异质活性 | Acid Phosphatase ≤2% of base activity |
| 储存温度 | −20℃ |
安全信息
| 储存分类代码 | 13 - Non Combustible Solids |
| WGK | WGK 3 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
