产品说明
一般描述
Two forms of Apo B exist: Apo B-100 and Apo B-48. The first is found in VLDL and LDL and is produced by the liver. The second is found in chylomicrons and originates in the intestine.
Apo B is purified from LDL isolated by density gradient ultracentrifugation (1.03-1.05 g/mL density range) followed by size exclusion chromatography in 10 mM deoxycholate, 50 mM NaCl, 50 mM Na2CO3, pH 10 (Walsh & Atkinson 1983).
PROPERTIES:
Apo B, which has a Mr of 550 kD, is the major protein found in LDL and is responsible for the cellular uptake of LDL particles from the plasma (Goldstein & Brown 1982). The Apo B prepared by this procedure is aggregated. The aggregates are not retained by a 0.45 mm filter, but migrate only a short distance into a 5% SDS PAGE gel. In the isolation buffer, Apo B gives a stable solution that is slightly hazy.
Product Source: Human plasma, fresh, non-frozen. Tested negative for anti-HIV-1, anti-HIV-2, anti-HCV, anti-HBc and HBsAg.
外形
Lyophilized from 50 mM Na2CO3, pH 10, 50 mM NaCl, and 10 mM sodium deoxycholate solution. Non-sterile.
储存及稳定性
Maintain lyophilized material at <= -20°C for up to six months. Reconstitute to 1 mL by adding deionized water. If the deoxycholate is removed, Apo B-100 will slowly aggregate and become insoluble. After reconstitution maintain at +2-8°C for up to 4 weeks.
分析说明
Single arc by IEP against antisera to whole human serum.
法律信息
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| eCl@ss | 32160405 |
| NACRES | NA.41 |
产品性质
| 质量水平 | 100 |
| 生物来源 | human |
| 测定 | >95% (SDS-PAGE) |
| manufacturer/tradename | Chemicon® |
| NCBI登记号 | NM_000384.2 |
| UniProt登记号 | P04114 |
| 运输 | dry ice |
| Gene Information | human ... APOB(338) |
安全信息
| 象形图 |  |
| 警示用语： | Warning |
| 危险声明 | H319 |
| 预防措施声明 | P264 - P280 - P305 + P351 + P338 - P337 + P313 |
| 危险分类 | Eye Irrit. 2 |
| 储存分类代码 | 11 - Combustible Solids |
| WGK | WGK 1 |
