Anti-ZO-1 Antibody, clone 5G6.1

产品说明

一般描述

Tight junction protein ZO-1 (UniProt Q07157; also known as Tight junction protein 1, Zona occludens protein 1, Zonula occludens protein 1) is encoded by the TJP1 gene (also known as ZO1) (Gene ID 7082) in human. The tight junction (TJ) constitutes the barrier between the apical and the basolateral domains of the plasma membrane. The assembly and permeability of this barrier are dependent on the zonula occludens (ZO) membrane-associated guanylate kinase (MAGUK) proteins ZO-1, ZO-2, and ZO-3. ZO-1, a 210-225 kDa protein, is found at the submembranous domain of TJs in epithelia and endothelia. ZO-1 contains three PDZ domains (PDZ1/aa23-109; PDZ2/aa181-261; PDZ3/aa422-502) at its N-treminal end, followed by an SH3 domain (aa519-580), a GUK/GK homology domain (aa632-782), an acidic domain (aa817-894), an alpha spliced domain (aa921-1000), and a C-terminal proline-rich/PR domain. ZO-1 is a phosphoprotein and a known substrate of serine/threonine kinases ZAK and of PKC. MAPK signaling pathway regulates tyrosine phosphorylation of ZO-1, and MEK1 inhibition in Ras transformed epithelial cells is reported to result in tyrosine phosphorylation of ZO-1 and occludin. ZO-1 interacts with claudins, JAM, ZO-2, and ZO-3 through its PDZ domains, while its GK module mediates interaction with occludin. ZO-1 also binds actin cytoskeleton and actin-binding protein 4.1 through its carboxyl terminal end. In addition, ZO-1 associates with AF-6 and cingulin at the TJ, as well as with the adherens junction protein alpha-catenin and with the gap junction proteins connexins 43 and 45.

免疫原

GST-tagged recombinant protein corresponding to human ZO-1.

应用

Research Category
Cell Structure
This Anti-ZO-1 Antibody, clone 5G6.1 is validated for use in Western Blotting, Immunocytochemistry, Flow Cytometry, ELISA for the detection of ZO-1 .
Research Sub Category
Adhesion (CAMs)
Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected ZO-1 in human and rat kidney tissues.

质量

Evaluated by Western Blotting in HCT116 cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected ZO-1 in 200 µg of HCT116 cell lysate.

目标描述

~195 kDa observed

外形

Purified mouse monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified
Protein G Purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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