Anti-ZEB2 Antibody

产品说明

一般描述

Zinc finger E-box-binding homeobox 2 (ZEB2), also known as Smad-interacting protein 1 (SIP1, SMADIP1) and Zinc finger homeobox protein 1b (ZFHX1B, ZFX1B), is a transcriptional inhibitor that binds to DNA sequence 5′-CACCT-3′ in different promoters and interacts with activated SMADs. ZEB2 has been shown to repress the transcription of the E-cadherin gene and induce epithelial-mesenchymal transition, a genetic program controlling cell migration during embryonic development and wound healing in vitro. It is suggested that ZEB2 contributes to tumor progression since it’s been demonstrated to protect cells from DNA damage-induced apoptosis. Recent studies have shown that ZEB2 is often observed in the cytoplasm in numerous cancer tissues, implying that its localization may be regulated in normal and tumor tissues. Mutations in this gene are also associated with Mowat-Wilson syndrome (MWIS), also known as Hirschsprung disease-mental syndrome.

免疫原

KLH-conjugated linear peptide corresponding to human ZEB2.

应用

Research Category
Cell Structure
Immunocytochemistry Analysis: 20 µg/mL from a representative lot detected ZEB2 in Jurkat cells.

Immunofluorescence Analysis: 20 μg/mL from a representative lot detected ZEB2 in Jurkat cells.
Detect ZEB2 using this rabbit polyclonal antibody, Anti-ZEB2 Antibody validated for use in western blotting, ICC & Immunofluorescence.
Research Sub Category
Adhesion (CAMs)

质量

Evaluated by Western Blotting in Jurkat cell lysate.

Western Blotting Analysis: 2 µg/mL of this antibody detected ZEB2 in 15 µg of Jurkat cell lysate.

目标描述

~200 kDa observed. Uncharacterized bands may appear in some lysate(s). Uniprot describes 2 isoforms at ~134 kDa and ~136 kDa The calculated molecular weight of this protein is 136 kDa, but can be observed at ~200 kDa due to post-translational modification.

外形

Purified rabbit polyclonal in buffer containing PBS with up to 0.1% sodium azide.
Antigen Affinity Purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

分析说明

Control
Jurkat cell lysate

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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