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Anti-WNT1 Antibody, clone 10C8

品牌
Sigma-Aldrich
货号
MABD168
规格纯度
ascites fluid, clone 10C8, from mouse
参考价格
5688.25 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

Wnt-1, also known as Proto-oncogene Wnt-1 or Proto-oncogene Int-1 homolog, was first identified as a proto-oncogene activated by viral insertion in mouse mammary tumors. The Wnt-1 gene encodes a member of a large family of secreted proteins that are cysteine-rich, glycosylated, and poorly soluble. Wnt-1 serves as a ligand for the RYK coreceptor, resulting in Wnt signaling. The Wnt signalling pathway mediates the levels of cytosolic beta-catenin, a multi-functional protein that associates with membrane-bound E-cadherin as well as several DNA-binding proteins, such as members of the TCF/LEF family. Additionally, it serves a ligand for members of the frizzled family of seven transmembrane receptors and plays a role in development of the central nervous system.

免疫原

Purified recombinant fragment of human WNT1 expressed in E. Coli.

应用

Immunofluorescence Analysis: A 1:200-1,000 dilution from a representative lot detected WNT1 in HeLa and 3T3-L1 cells.

Immunohistochemistry Analysis: A 1:200-1,000 dilution from a representative lot detected WNT1 in human adrenal tissue.

Flow Cytometry Analysis: A 1:200-400 dilution from a representative lot detected WNT1 in HeLa cells.

Optimal working dilutions must be determined by end user.


Research Sub Category
Pluripotent & Early Differentiation
Detect Wnt-1 using this mouse monoclonal antibody, Anti-WNT1 Antibody, clone 10C8 validated for use in western blotting, Immunofluorescence, IHC & Flow Cytometry.
Research Category
Stem Cell Research

质量

Evaluated by Western Blotting in NIH/3T3, 3T3L1, and HeLa cell lysates.

Western Blot Analysis: A 1:500-2,000 dilution of this antibody detected WNT1 in NIH/3T3, 3T3L1, and HeLa cell lysates.

目标描述

~41 kDa observed. Uncharacterized bands may appear in some lysate(s).

外形

Unpurified
Mouse monoclonal IgG1 ascitic fluid containing up to 0.1% sodium azide.

储存及稳定性

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

分析说明

Control
NIH/3T3, 3T3L1, and HeLa cell lysates

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss32160702
NACRESNA.41

产品性质

质量水平100
生物来源mouse
抗体形式ascites fluid
antibody product typeprimary antibodies
克隆10C8, monoclonal
species reactivitymouse, human
technique(s)flow cytometry: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
western blot: suitable
同位素/亚型IgG1
UniProt登记号P04628
运输wet ice
Gene Informationhuman ... WNT1(7471)

安全信息

储存分类代码12 - Non Combustible Liquids
WGKnwg
闪点(F)Not applicable
闪点(C)Not applicable

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