产品说明
一般描述
WIPI-2, also known as Atg18, is involved in autophagy by regulating the assembly of mulitprotein complexes by presenting a 7-bladed propeller structure for interactions with phospholipids. Atg18 is a mammalian effector of PtdIns3P and is ubiquitously expressed in a variety of cell lines. Studies show that Atg18 is recruited to early autophagosomal structural formation along with Atg16 and ULK1, and Atg18 is required for the formation of LC3-positive autophagosomes.
免疫原
GST-tagged recombinant protein corresponding to the C-terminus of human WIPI-2.
Epitope: C-terminus
应用
Research Sub Category
Apoptosis - Additional
This Anti-WIPI-2, clone 1B8.1 Antibody is validated for use in Western Blotting and Immunohistochemistry (Paraffin) for the detection of WIPI-2.
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected WIPI-2 in human pancreas tissue.
Research Category
Apoptosis & Cancer
质量
Evaluated by Western Blotting in human testis tissue lysate.
Western Blotting Analysis: 2.0 µg/mL of this antibody detected WIPI-2 in 10 µg of human testis tissue lysate.
目标描述
~49 kDa observed. Uncharacterized band(s) may appear in some lysates.
外形
Format: Purified
Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Protein G Purified
储存及稳定性
Stable for 1 year at 2-8°C from date of receipt.
其他说明
Concentration: Please refer to lot specific datasheet.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
产品性质
质量水平 | 100 |
生物来源 | mouse |
抗体形式 | purified immunoglobulin |
antibody product type | primary antibodies |
克隆 | 1B8.1, monoclonal |
species reactivity | human |
technique(s) | immunohistochemistry: suitable (paraffin) western blot: suitable |
同位素/亚型 | IgG2aκ |
NCBI登记号 | NP_001028690 |
UniProt登记号 | Q9Y4P8 |
运输 | wet ice |
Gene Information | human ... WIPI2(26100) |
安全信息
储存分类代码 | 12 - Non Combustible Liquids |
WGK | WGK 1 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |