产品说明
一般描述
Monoclonal Anti-VPS4A (mouse IgG1 isotype) is derived from the hybridoma VPS4-110 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to a fragment of human VPS4A, conjugated to KLH.
Vacuolar-protein-sorting factor 4A (VPS4A) is an AAAtype ATPase, encoded by the gene mapped to human chromosome 16q22.1. The encoded protein is a member of vacuolar sorting protein family. The protein is characterized by a canonical AAA ATPase cassette followed by an N-terminal MIT (microtubule interacting and trafficking) domain.
应用
Monoclonal Anti-VPS4A antibody has been used in
- immunoblotting
- immunoprecipitation
- immunolabelling
生化/生理作用
Vacuolar-protein-sorting factor 4A (VPS4A) plays a vital role in lysosomal/endosomal membrane trafficking. It is also involved in the regulation of exosome biogenesis. Downregulated expression of the gene leads to the development of hepatocellular carcinoma (HCC). Thus, VPS4 is considered to be an effective tumor suppressor for HCC. Mutation of the gene leads to defective multivesicular body formation.
外形
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide as a preservative.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| NACRES | NA.41 |
产品性质
| 生物来源 | mouse |
| 偶联物 | unconjugated |
| 抗体形式 | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| 克隆 | VPS4-110, monoclonal |
| 形式 | buffered aqueous solution |
| 分子量 | antigen ~50 kDa |
| species reactivity | mouse, bovine, canine, human, rat |
| 浓度 | ~1.0 mg/mL |
| technique(s) | immunoprecipitation (IP): suitable western blot: 1-2 μg/mL using whole extracts of mouse 3T3 or human HEK-293 cells. |
| 同位素/亚型 | IgG1 |
| UniProt登记号 | Q9UN37 |
| 运输 | dry ice |
| 储存温度 | −20℃ |
| Gene Information | human ... VPS4A(27183) mouse ... Vps4a(116733) rat ... Vps4a(246772) |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | WGK 1 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
