Anti-VE-Cadherin Antibody, clone Vli37

产品说明

一般描述

Cadherin-5 (UniProt P55284; also known as Vascular endothelial cadherin, VE-Cad, VE-cadherin, VEcad, CD144) is encoded by the Cdh5 gene (Gene ID 12562) in murine species. Cadherins (calcium-dependent adhesion) are type-1 transmembrane proteins that form adherens junctions in tissues and play important roles in mediating cell adhesion. Cadherins are designated with a prefix that specifies their tissue association. Vascular endothelial-cadherin (VE-cadherin) is the transmembrane component of the endothelial adherens junction between vascular endothelial cells (ECs) and plays a pivotal role in endothelium integrity and in the control of vascular permeability. One characteristic of VEGF-induced vascular permeability is the phosphorylation of VE-cadherin, which leads to VE-cadherin internalization and the destabilization of adherens junctions. Likewise, VE-cadherin overexpression is shown to decrease the permeability of endothelial monolayers in vitro. VE-cadherin is initially produced with a signal peptide (a.a. 1-24) and a propeptide (a.a. 25-45) sequence, the removal of which yields tthe mature protein containing a large extracellular region (a.a. 46-599) with five cadherine repeats (a.a. 46-149, 150-256, 257-371, 372-476, 477-593), followed by a transmembrane segment (a.a. 600-620) and a cytoplasmic domain (a.a. 621-784).

特异性

Clone Vli37 stains endothelial adherens junctions by immunocytochemistry and immunohistochemistry.

免疫原

Epitope: Near C-terminus.
Linear peptide corresponding to the C-terminal cytoplasmic domain sequence of mouse VE-Cadherin.

应用

Research Category
Cell Structure
Western Blotting Analysis: An 1:500 dilution of this antibody detected VE-Cadherin in 10 µg of mouse brain endothelial bEnd.3 cell lysate.
Western Blotting Analysis: An 1:2,000 dilution from a representative lot detected VE-cadherin expression in lysastes from mouse lung tissue, mouse brain endothelial bEnd.3 cells, and bovine aortic endothelial GM7372 cells (Courtesy of Dr. Volkhard Lindner, Maine Medical Center Research Institute, Scarborough, ME).
Immunohistochemistry Analysis: An 1:250-5,000 dilution from a representative lot detected VE-cadherin immunoreactivity in formalin-fixed, paraffin-embeded mouse lung, aorta, and liver tissue sections (Courtesy of Dr. Volkhard Lindner, Maine Medical Center Research Institute, Scarborough, ME).
Immunocytochemistry Analysis: An 1:500-5,000 dilution from a representative lot detected VE-cadherin immunoreactivity in bovine aortic endothelial GM7372 cells (Courtesy of Dr. Volkhard Lindner, Maine Medical Center Research Institute, Scarborough, ME).
This Anti-VE-Cadherin Antibody, clone Vli37 is validated for use in Western Blotting, Immunohistochemistry (Paraffin), Immunocytochemistry for the detection of VE-Cadherin.
Research Sub Category
Adhesion (CAMs)

质量

Evaluated by Western Blotting in mouse lung tissue lysate.

Western Blotting Analysis: An 1:500 dilution of this antibody detected VE-Cadherin in 10 µg of mouse lung tissue lysate.

目标描述

~110 observed. Target band size appears larger than the calculated molecular weight of 83.05 kDa due to glycosylation.

外形

Unpurified
Rabbit monoclonal IgG in supernatant with 0.05% sodium azide.

储存及稳定性

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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