Anti-Tumor Necrosis Factor-β Antibody, clone 9B9

产品说明

特异性

This antibody is use for detection, identification and neutralixzation of TNF-b in cell culture and other biological samples. The antibody reacts with human natural and recombinant TNF-b (lymphotoxin). It does not react with human TNF-a. Determination of cross-reactivity to TNF-b from other species has not been done.

免疫原

Human, recombinant tumor necrosis factor-beta (TNF-beta, lymphotoxin) produced in E. Coli.

应用

Anti-Tumor Necrosis Factor-β Antibody, clone 9B9 detects level of Tumor Necrosis Factor-β & has been published & validated for use in IC, IH & WB.
Western blot

Immunocytochemistry

Immunohistochemistry

Neutralization: 200ng of TNF-beta antibody is required for the neutralizaion of 1 unit/ml TNF-beta. The TNF-beta-preparation which is used has a specific activity of ca. 2x10(7) units/mg protein [cell lytic assay with L 929 cells (mouse transformed fibroblasts) and/or WEHJ 164 cells (mouse fibrosarcoma cells)/actinomycin D] .

Optimal working dilutions must be determined by end user.

外形

Purified immunoglobulin (Protein A-agarose chormotography). One bottle of lyophilized preparation contains:200ug mouse IgG, 2 mg potassium phosphate buffer, 2.9 mg sodium chloride, and 20 mg raffinose.Reconstitution with 1 ml sterile, distilled water results in an antibody concentration of 200 ug/ml.
Format: Purified

储存及稳定性

Maintain frozen at -20°C in undiluted aliquots for up to 12 months.Freeze only once.

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

产品性质

安全信息