产品说明
一般描述
Anti-Tubulin, Tyrosine antibody, Mouse monoclonal (mouse IgG3 isotype) is derived from the TUB-1A2 hybridoma, produced by the fusion of mouse myeloma cells and splenocytes from mouse. Tubulin is an intracellular cylindrical filamentous structure and is presented in eukaryotic cells. It consists of three isoforms α-, β- and γ-tubulin. Tubulin is a heterodimer of α- and β- tubulin subunits.
特异性
Anti-Tubulin, Tyrosine antibody, Mouse monoclonal specifically recognizes the C-terminal Tyrosine of Tubulin protein. The antibody is non-reactive with cells that have been treated with pancreatic carboxypeptidase A under conditions which remove only the C-terminal tyrosine. Monoclonal Anti-Tubulin, Tyrosine recognizes Tubulin from human, mouse, chicken, bovine, rat, hamster, canine, monkey, porcine, arabidopsis, tobacco, yeast, Xenopus, insect, comb jelly, sponge, and worm origin.
免疫原
a synthetic peptide containing the carboxy terminal amino acids of porcine a-tubulin
应用
Anti-Tubulin, Tyrosine antibody, mouse monoclonal may be used in:
- immunoblotting
- immunofluorescence
- immunohistochemistry
生化/生理作用
Tubulin is the major building block of microtubules which is essential for structural and mobility elements in mitosis, intracellular transport, flagellar movement and in the cytoskeleton. It is also involved in the nervous system. Tubulin is associated with neurodegenerative disorders such as Alzheimer′s and Parkinson′s disease. Mutations in the αβ- dimers and γ-tubulin are associated with malformation and impairment of cognitive disorders.
外形
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
储存及稳定性
For continuous use, store at 2–8 ℃ for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
免责声明
Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| NACRES | NA.41 |
产品性质
| 质量水平 | 200 |
| 生物来源 | mouse |
| 抗体形式 | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| 克隆 | TUB-1A2, monoclonal |
| 形式 | buffered aqueous solution |
| 分子量 | ~55 kDa |
| species reactivity | monkey, hamster, human, yeast, canine, Arabidopsis sp., Xenopus, chicken, mouse, bovine, insect, rat, porcine |
| 浓度 | ~1 mg/mL |
| technique(s) | immunoblotting: suitable immunofluorescence: 2-4 μg/mL using human HeLa or Chicken fibroblast cells immunohistochemistry: suitable |
| 同位素/亚型 | IgG3 |
| UniProt登记号 | Q71U36 |
| 运输 | dry ice |
| 储存温度 | −20℃ |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | WGK 1 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
