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Anti-TRP1/TYRP1 Antibody, clone TA99, Azide Free

品牌
Sigma-Aldrich
货号
MABC592
规格纯度
clone TA99, 1 mg/mL, from mouse
参考价格
6022.61 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

TRP1/TYRP1, also known as 5,6-dihydroxyindole-2-carboxylic acid oxidase, DHICA, Catalase B, Glycoprotein 75, Melanoma antigen gp75, or Tyrosinase-related protein 1 (TRP/TRP-1/TRP1), and encoded by the gene TYRP1/CAS2/TYRP/TYRRP, is involved in melanin synthesis and is the enzyme that oxidizes DHICA into indole-5,6-quinone-2-carboxylic acid in the pathway. TRP1/TYRP1 is critical for skin/eye/hair pigmentation. Mutations are associated with Albinism. TRP1/TYRP1 is regulated by the microphthalmia-associated transcription factor (MITF). MITF is a master regulator of pigmentation and a target of β-catenin in Wnt/β-catenin signaling during melanocyte differentiation. TRP1/TYRP1 is also regulated by the microRNA miR-145, and this microRNA is being examined as a possible therapy for hyperpigmentation. TRP1/TYRP1 is expressed as a membrane protein in melanosomes and endosomes in pigment cells.

免疫原

Whole cells corresponding to human TRP1/TYRP1.

应用

Research Sub Category
Apoptosis - Additional
This Anti-TRP1/TYRP1 Antibody, clone TA99, Azide Free is validated for use in western blotting, IHC, blocking of inteferring antibodies, IP & flow cytometry for the detection of TRP1/TYRP1.
Research Category
Apoptosis & Cancer
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected TRP1/TYRP1 in human melanoma tissue.

Blocking of Interferring Antibodies Analysis: A representative lot from an independent laboratory suppressed the growth of subcutaneous B16 tumors (Patel, D., et al. (2008). Anticancer Res. 28(5A):2679-2686.).

Activity Assay Analysis: A representative lot from an independent laboratory improves anti-tumor efficacy by augmenting systemic CD8+T cell responses to tumor cells (Saenger, Y. M., et al. (2008). Cancer Res. 68(23):9884-9891.).

Immunoprecipiptation Analysis: A representative lot from an independent laboratory immunoprecipiated TRP1/TYRP1 from B16 cell lysate (Srinivasan, R., et al. (2002). Cancer Immun. 19(2):8.).

质量

Evaluated by Western Blottng in mouse skin tissue lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected TRP1/TYRP1 in 10 µg of mouse skin tissue lysate.

目标描述

~60 kDa observed

外形

Purified mouse monoclonal IgG2aκ in buffer containing PBS without preservatives.
Format: Purified
Protein G Purified

储存及稳定性

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss32160702
NACRESNA.41

产品性质

质量水平100
生物来源mouse
抗体形式purified immunoglobulin
antibody product typeprimary antibodies
克隆TA99, monoclonal
species reactivitymouse, human
浓度1 mg/mL
technique(s)blocking: suitable (interfering antibodies)
flow cytometry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
同位素/亚型IgG2aκ
NCBI登记号NP_000541
UniProt登记号P17643
运输wet ice

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 1
闪点(F)Not applicable
闪点(C)Not applicable

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