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Anti-Trop2, clone 2G10, Azide Free Antibody

品牌
Sigma-Aldrich
货号
MABC519
规格纯度
clone 2G10, from mouse
参考价格
6022.61 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

The protein known as Trop-2, or also known as cell surface glycoprotein Trop-2, Membrane component chromosome 1 surface marker 1 or Pancreatic carcinoma marker protein GA733-1 and encoded by the gene TACSTD2/GA733-1/M1S1/TROP2, is a protein that may function as a growth factor receptor and is expressed primarily in the placenta, lung and epithelial tissues but also found in certain cancers. The protein was first identified as a carcinoma antigen by the monoclonal GA733. It is a plasma membrane bound protein that transduces an intracellular calcium signal after binding unknown ligands. Mutations in Trop2 are associated with a rare eye disease leading to blindness. Trop-2 has also recently been shown to be important in regulating fibroblast motility and lung cell proliferation via the regulation of the ERK kinase pathway as well as several critical components of the cell migration apparatus.

免疫原

Recombinant protein corresponding to the extracellular domain of human Trop2.

应用

Research Sub Category
Apoptosis - Additional
This Anti-Trop2 antibody, clone 2G10, Azide Free is validated for use in ICC, flow cytometry & inhibition for the detection of Trop2.
Research Category
Apoptosis & Cancer
Immunocytochemistry Analysis: A representative lot detected Trop2 in MTE 4-14/T2 and MCF7 cells (Prof. Saverio Alberti, Oncoxx Biotec.).

Flow Cytometry Analysis: A representative lot detected Trop2 in transfected cells, Skov-3 cancer cells, and HT29 cancer cells (Prof. Saverio Alberti, Oncoxx Biotec.).

Inhibition Analysis: A representative lot inhibited tumor growth in DU145 prostate cancer cells, and in HT-29, HCT116 and Colo205 cells. (Note: When used together, Cat. No. MABC519, Anti-Trop2, clone 2G10, Azide Free and Cat. No. MABC518, Anti-Trop2, clone 2EF, Azide Free maximizes inhibition of tumor growth in vivo.) (Prof. Saverio Alberti, Oncoxx Biotec.).

质量

Evaluated by Immunocytochemistry Analysis in MET cells transfected with a vector expressing Trop2 or with an empty vector.

Immunocytochemistry Analysis: A 1:50 dilution of this antibody detected Trop 2 in MET cells transfected with a vector expressing Trop2 and not in MET cells transfected with an empty vector.

目标描述

36 kDa calculated

外形

Purified mouse monoclonal IgG2aκ in buffer containing PBS without preservatives.
Format: Purified
Protein G Purified

储存及稳定性

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss32160702
NACRESNA.41

产品性质

质量水平100
生物来源mouse
抗体形式purified immunoglobulin
antibody product typeprimary antibodies
克隆2G10, monoclonal
species reactivityhuman
technique(s)flow cytometry: suitable
immunocytochemistry: suitable
inhibition assay: suitable
同位素/亚型IgG2aκ
NCBI登记号NP_002344
UniProt登记号P09758
运输wet ice
Gene Informationhuman ... TACSTD2(4070)

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 1
闪点(F)Not applicable
闪点(C)Not applicable

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