Anti-trimethyl Histone H3 (Lys36) Antibody, clone RM155

产品说明

一般描述

Information Histone H3, also known as Histone H3.1t (H3/t), H3t, H3/g, and encoded by the gene name HIST3H3/ H3FT, is a core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Therefore, histones play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine. High levels of phosphorylation of Histone H3 are associated with mitosis.

特异性

This antibody reacts to Histone H3 trimethylated at Lysine 36 (K36me3). No cross reactivity with monomethylated Lysine 36 (K36me1), dimethylated Lysine 36 (K36me2), or other methylations in Histone H3.

免疫原

A linear peptide corresponding to human Histone H3 trimethylated at Lys36.

应用

This Anti-trimethyl Histone H3 (Lys36) Antibody, clone RM155 is validated for use in Western Blotting, Multiplexing for the detection of trimethyl Histone H3.

质量

Evaluated by Western Blotting in HeLa acid extract.

Western Blotting Analysis (WB): 2 μg/mL of this antibody detected trimethyl Histone H3 (Lys36) in Recombinant HeLa protein and HeLa acid extract.

目标描述

~17 kDa observed. Uncharacterized bands may be observed in some lysate(s).

外形

Protein A purified
Format: Purified

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

产品性质