Anti-TRIM33 Antibody, clone 3B5.1

产品说明

一般描述

TRIM33, also known as E3 ubiquitin-protein ligase TRIM33, Ectodermin homolog, RET-fused gene 7 protein, Rfg7, Transcription intermediary factor 1-gamma, TIF1-gamma, or Tripartite motif-containing protein 33, and encoded by the gene TRIM33/KIAA1113/RFG7/TIF1G, is an E3 ubiquitin protein ligase. Among its targets is SMAD4 for which ubiquitination facilitates its nuclear exclusion and degradation. SMAD3 is also a target for ubiquitination, and together, TRIM33′s association promotes erythroid differentiation of hematopoietic stem cells. Finally mono-ubiquitination of various targets by TRIM33 affects their ability to form complexes which often hampers their activity and thus regulates their subsequent actions. Such is the case of SMAD4 and its interaction with TGF-beta/BMP signaling. Monoubiquitination of SMAD4 by TRIM33 hampers its ability to form a stable complex with activated SMAD2/3 resulting in inhibition of TGF-beta/BMP signaling cascade. TRIM33 is localized in nucleus in nuclear dots or bodies and is expressed in most tissues and stem cells during development as well as in the adult in multiple tissues.

免疫原

GST-tagged recombinant protein corresponding to human TRIM33.

应用

Research Sub Category
Nuclear Receptors
This Anti-TRIM33 Antibody, clone 3B5.1 is validated for use in Western Blotting and Immunocytochemistry for the detection of TRIM33.
Research Category
Epigenetics & Nuclear Function
Immunocytochemistry Analysis: A 1:50 dilution from a representative lot detected TRIM33 in H9 stem cells.
Alexa Fluor^® is a registered trademark of Life Technologies.

质量

Evaluated by Western Blotting in K562 cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected TRIM33 in 10 µg of K562 cell lysate.

目标描述

~123 kDa observed. Uncharacterized band(s) may appear in some lysates.

外形

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified
Protein G Purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

法律信息

ALEXA FLUOR is a registered trademark of Life Technologies

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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