产品说明
一般描述
TRIM5 alpha Antibody: TRIM5 is a member of a broad family of otherwise unrelated proteins defined by the presence of a tripartite motif containing a RING domain, a B-box type 1, and a B-box type 2, followed by a coiled-coil region. TRIM5 has six alternately spliced isoforms, the longest of which is the alpha variant which also contains a carboxy-terminal B30.2 (SPRY) domain. Expression of TRIM5α variants from humans, rhesus monkeys, and African green monkeys enabled resistance to infection by various retroviruses including HIV-1, albeit at differing efficiencies. All TRIM5α variants could inhibit at least two different retroviruses, but not from those viruses isolated from the same species, suggesting that TRIM5α acts as a natural barrier to cross-species retrovirus transmission.
免疫原
TRIM5 alpha antibody was raised against a synthetic peptide corresponding to amino acids near the C-terminus of rhesus monkey TRIM5 alpha.The immunogen is located within the last 50 amino acids of TRIM5 alpha.
应用
TRIM5 alpha antibody can be used for detection of TRIM5 alpha by Western blot at 2 μg/mL. Antibody can also be used for immunoflourescence starting at 10 μg/mL. For immunofluorescence start at 10 μg/mL.Antibody validated: Western Blot in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
外形
Supplied in PBS containing 0.02% sodium azide.
产品性质
| 生物来源 | rabbit |
| 质量水平 | 100 |
| 偶联物 | unconjugated |
| 抗体形式 | affinity isolated antibody |
| antibody product type | primary antibodies |
| 克隆 | polyclonal |
| 形式 | liquid |
| species reactivity | human |
| 浓度 | 1 mg/mL |
| technique(s) | immunofluorescence: suitable western blot: suitable |
| 同位素/亚型 | IgG |
| UniProt登记号 | Q0PF16 |
| 运输 | wet ice |
| 储存温度 | −20℃ |
| Gene Information | rhesus macaque ... TRIM5(574288) |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | nwg |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
