Anti-THAP11 Antibody

产品说明

一般描述

THAP11 (Ronin) is a transcriptional repressor which is predominantly expressed in embryonic stem (ES) cells. It contains a THAP-type zinc finger domain which is characteristic of the THAP 11 family members. In ES cells, THAP11 interacts with the transcriptional co-regulator HCF-1, to recruit chromatin-modifying proteins such as members of the polycomb family of proteins, to induce gene repression. THAP11 targets genes involved in metabolism, transcriptional initiation, and messenger RNA splicing. It plays an important role in maintaining the pluripotent state of ES cells.

免疫原

Recombinant protein corresponding to human THAP11.

应用

Research Sub Category
Transcription Factors
Western Blotting Analysis: A representative lot of an affinity purified version of this antibody detected THAP11 in tested cell lysates (Parker, J. B., et al. (2012). Mol Cell Biol. 32(9):1654-1670.).

Immunoprecipitation Analysis: A representative lot of an an affinity purified version of this antibody immunoprecipitated THAP11 in tested cell lysates (Parker, J. B., et al. (2012). Mol Cell Biol. 32(9):1654-1670.).

Chromatin Immunoprecipitation Analysis: A representative lot of an an affinity purified version of this antibody immunoprecipitated THAP11 in SW620 cell lysates (Parker, J. B., et al. (2012). Mol Cell Biol. 32(9):1654-1670.).

Immunohistochemistry Analysis: A representative lot of an an affinity purified version of this antibody detected THAP11 in normal colon epithelium and colon adenocarcinoma tissues, and in lymph node metastasis tissues (Parker, J. B., et al. (2012). Mol Cell Biol. 32(9):1654-1670.).
Research Category
Epigenetics & Nuclear Function
Anti-THAP11 Antibody is a highly specific rabbit polyclonal antibody, that targets Ronin & has been tested in western blotting, IP & IHC.

质量

Evaluated by Western Blotting in HUVEC cell lysate.

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected THAP11 in 10 µg of HUVEC cell lysate.

目标描述

~45 kDa observed. Uniprot describes a molecular weight at 35 kDa However, this protein may be observed at ~45 kDa upon forming a complex with HCF-1. (Parker, J. B., et al. (2012). Mol Cell Biol 32(9):1654-1670.; Spanu, P. (1997). Gene. 193(1):89-96.).

外形

Unpurified
Flow through from affinity depleted rabbit polyclonal serum containing 0.05% sodium azide.

储存及稳定性

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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