Anti-TBX1 Antibody, clone 7E12.1

产品说明

一般描述

T-box transcription factor TBX1 (UniProt O43435; also known as T-box protein 1, Testis-specific T-box protein) is encoded by the TBX1 (also known as CTHM, DGS, TBX1C, VCFS) gene (Gene ID 6899) in human. TBX1 blongs to the T-box family of transcription factors that play important roles in the formation of tissues and organs during embryonic development. TBX1 is required for the second heart field (SHF) development for outflow tract development by regulating proliferation and differentiation of SHF cells. TBX1 is also shown to negatively regulate thymic epithelial cell (TEC) growth and differentiation in mice, extinction of Tbx1 gene expression in the third pharyngeal pouch (3rd pp) endoderm is a prerequisite for thymus organogenesis. Human TBX mutations cause DiGeorge syndrome, characterized by parathyroid hypoplasia, thymic hypoplasia, defects in the outflow tract of the heart, and craniofacial anomalies.

特异性

Clone 7E12.1 was raised against a recombinant fragment derived from TBX1 spliced isoform A (Uniprot O43435-1), cross-reactivity toward spliced isoform B and isoform C (TBX1C) is possible, but has not been determined.

免疫原

Epitope: C-terminal region.
GST-tagged recombinant human TBX1 C-terminal fragment.

应用

Research Sub Category
Transcription Factors
Anti-TBX1 Antibody, clone 7E12.1 is an antibody against TBX1 for use in Western Blotting.
Western Blotting Analysis: A 1:500 dilution from a representative lot detected TBX1 in 10 µg of mouse Neuro-2a and human SH-SY5Y neuroblastoma cell lysates.
Research Category
Neuroscience

质量

Evaluated by Western Blotting in HUVEC lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected TBX1 in 10 µg of HUVEC lysate.

目标描述

~48 kDa observed. 43.13 kDa (isoform A), 40.35 kDa (isoform B), and 52.67 kDa (isoform C; TBX1C) calculated. Uncharacterized band(s) may appear in some lysates.

联系

Replaces: AB15624

外形

Unpurified
Mouse monoclonal IgG2aκ hybridoma culture supernatant without preservatives.

储存及稳定性

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

产品性质

安全信息