Anti-Tapasin Antibody, clone 7F6

产品说明

一般描述

Tapasin (TPN or TPSN) is also known as NGS-17, TAP-associated protein and TAP-binding protein. Tapasin plays a role in MHC class I peptide loading and assembly, and is involved in associating MHC class I with transporter associated with antigen processing (TAP). Tapasin is found in neutrophils, predominantly within fully differentiated cells.

特异性

This antibody recognizes the Lumenal domain of Tapasin.

免疫原

Linear peptide corresponding to human Tapasin.

应用

Detect Tapasin using this rat monoclonal antibody, Anti-Tapasin Antibody, clone 7F6 validated for use in western blotting & Flow Cytometry.
Western Blotting Analysis: 0.5 µg/mL from a representative lot detected Tapasin in 10 µg of A431, HEK293, HepG2, HUVEC, Jurkat, and PC12 cell lysates.

Flow Cytometry Analysis: 4 µg from a representative lot detected Tapasin in 1X10E6 PBMC cells.

Western Blotting Analysis: A representative lot from an independent laboratory detected Tapasin in BNLF2aHA transfected MJS cell lysate (Hislop, A. D., et al. (2007). J Exp Med. 204(8):1863-1873.).

Western Blotting Analysis: A representative lot from an independent laboratory detected Tapasin in HeLa cell lysate (Hulpke, S., et al. (2012). Cell Mol Life Sci. 69(19):3317-3327.).

Western Blotting Analysis: A representative lot from an independent laboratory detected Tapasin in BNLF2aHA4M transfected MJS cell lines (Horst, D., et al. (2009). J Immunol. 182(4):2313-2314.).

Western Blotting Analysis: A representative lot from an independent laboratory detected Tapasin in WB (Koch, J., et al. (2006). FEBS Lett. 580(17):4091-4096.).

质量

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected Tapasin in 10 µg of HeLa cell lysate.

目标描述

~48 kDa observed

外形

Protein G Purified
Format: Purified

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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