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Anti-Substance P Receptor

品牌
Sigma-Aldrich
货号
ABN1369
规格纯度
from rabbit, purified by affinity chromatography
参考价格
5352.52 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

Substance-P receptor (UniProt P25103; also known as Neurokinin-1 receptor, NK-1 receptor, NK-1R, SPR, Tachykinin receptor 1) is encoded by the TACR1 (also known as NK1R, TAC1R) gene (Gene ID 6879) in human. NK-1, NK-2 and NK-3 are receptors that mediate the biological activities of tachykinins, including substance P (SP), neurokinin A (NKA), neurokinin B (NKB), ranakinin, kassinin, neuropeptide gamma, and eledoisin, widely distributed in the central and peripheral nervous systems. SP shows the highest affinity toward NK-1R (substance-P receptor) and exerts its biological activities via NK-1R, including addiction, depression, chronic inflammation, and cancer progression. The involvement of NK-1R in acute pancreatitis through mediating neurogenic inflammation has also been reported. Upon stimulation by SP, NK-1R interacts with multiple G proteins, including G s, G q/11, G i/o, G 12, and G 13, generating second messengers and activating downstream effector molecules. After binding NK-1R, SP induces a clathrin-dependent internalization of the receptor-ligand complex, SP is then degraded and the receptor is recycled to the cell surface. NK-1R is a 7-transmembrane (32-54, 65-86, 107-128, 149-169, 195-219, 249-270, 284-308) GPCR, having its N-terminal end (a.a. 1-31) at the extracelluar side and C-terminal end (a.a. 309-407) at the cytoplasmic side. There exists a second alternatively spliced human NK-1R isoform that lacks the C-terminal cytoplasmic domain (a.a. 312-407 missing). The full-length form enhances the growth of cancer cells and stimulates the production of cytokines, which in turn up-regulate the truncated form. The truncated form mediates malignancy and is more abundant in colonic epithelial cells from patients with colitis-associated cancer. In cancer cells, the NK-1 receptor is mainly localized on the plasma membrane and in cytoplasm, and occasionally also in the nucleus.

特异性

This rabbit polyclonal antibody targets substance P receptor C-terminal end sequence. Target region is absent in human spliced isoform 2.

免疫原

KLH-conjugated linear peptide corresponding to the C-terminal end sequence of human substance P receptor.

应用

Detect Substance P receptor using this rabbit polyclonal Anti-Substance P Receptor Antibody, Cat. No. ABN1369, validated for use in Immunohistochemistry and Western Blotting.
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected substance P receptor in human, mouse, and rat brain tissue sections.

质量

Evaluated by Western Blotting in rat brain tissue lysate.

Western Blotting Analysis: 0.2 µg/mL of this antibody detected substance P receptor in 10 µg of rat brain tissue lysate.

目标描述

~58 kDa observed. Target band size appears larger than the calculated molecular weights of 46.25/46.32/46.37 kDa (human/mouse/rat) due to glycosylation. Uncharacterized bands may be observed in some lysate(s).

外形

Affinity purified.

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss32160702

产品性质

生物来源rabbit
质量水平100
抗体形式affinity isolated antibody
antibody product typeprimary antibodies
克隆polyclonal
纯化方式affinity chromatography
species reactivitymouse, human, rat
species reactivity (predicted by homology)rhesus macaque (based on 100% sequence homology), canine (based on 100% sequence homology)
technique(s)immunohistochemistry: suitable
western blot: suitable
NCBI登记号NP_001049
UniProt登记号P25103
运输ambient

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 1
闪点(F)Not applicable
闪点(C)Not applicable

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