产品说明
一般描述
Ser-Arg-rich (SR) proteins make up a family of functionally and structurally conserved phosphoproteins, and are required components of alternative and constitutive pre-mRNA splicing. SR proteins are characterized by their modular composition consisting of two domains of interest: an N-terminal RNA recognition motif (RRM) which serves in the determination of DNA-binding specificity, and the RS domain, an arginine and serine rich, C-terminal domain that serves as a shuttling and localization director of SR proteins and as a splicing activation domain. They are involved in various aspects of pre-mRNA splicing and in spliceosome assembly including the identification and appropriation of splice-sites and the manipulation of alternative splicing regulation.
特异性
This antibody recognizes the four nuclear proteins SRp75, SRp55, SRp40, and SRp20, plus ~20 other prominent nuclear proteins believed to correspond to the SR family.
免疫原
Dephosphorylated bovine SRp55 and GST-tagged recombinant dephosphorylated SRp55 (Neugebauer, KM et. al 1995).
应用
Immunoprecipitation Analysis: A representative lot was used by an independent laboratory in IP (Neugebauer, KM, et al. (1995).
Immunocytochemistry Analysis: A representative lot was used by an independent laboratory in IC. (Neugebauer, KM, et al. (1995).
Anti-SR protein family Antibody, clone 16H3 is a Mouse Monoclonal Antibody for detection of SR protein family also known as arginine/serine-rich splicing factor 6 & has been validated in WB, IP, ICC.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
RNA Metabolism & Binding Proteins
质量
Evaluated by Western Blot in HeLa nuclear extract.
Western Blot Analysis: 0.5 µg/mL of this antibody detected SR proteins in 10 µg of HeLa nuclear extract.
目标描述
Multiple SR protein family members detected. Reported to predominantly detect SRp75, SRp55, SRp40, SRp30, SRp20 (Neugebauer, KM, et al. (1995).
外形
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified
Protein G Purified
储存及稳定性
Stable for 1 year at 2-8°C from date of receipt.
分析说明
Control
HeLa nuclear extract
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| eCl@ss | 32160702 |
| NACRES | NA.41 |
产品性质
| 质量水平 | 100 |
| 生物来源 | mouse |
| 抗体形式 | purified immunoglobulin |
| antibody product type | primary antibodies |
| 克隆 | 16H3, monoclonal |
| species reactivity | human |
| species reactivity (predicted by homology) | mouse (based on 100% sequence homology), rat (based on 100% sequence homology) |
| technique(s) | immunocytochemistry: suitable immunoprecipitation (IP): suitable western blot: suitable |
| 同位素/亚型 | IgG1κ |
| NCBI登记号 | NP_006266 |
| UniProt登记号 | Q13247 |
| 运输 | wet ice |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | WGK 1 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
