产品说明
一般描述
Anti-Splicing Factor SC-35 antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the SC-35 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a RBF-DHJ mouse immunized with partially purified spliceosomes prepared from HeLa cells. Splicing Factor SC-35, also known as serine/arginine rich splicing factor 2 (SRSF2) belongs to the family of of serine/arginine-rich (SR) proteins.
免疫原
partially purified spliceosomes prepared from HeLa cells
应用
Anti-Splicing Factor SC-35 antibody has been used in
- dot blot
- enzyme linked immunosorbent assay (ELISA)
- immunofluorescence
- immunohistochemistry
- immunoblotting
- immunoprecipitation
生化/生理作用
Splicing Factor SC-35 are critical regulators of constitutive and alternative pre-mRNA splicing. It also controls cellular apoptosis in response to genotoxic stress. Serine/arginine protein kinase 1 (SRPK1) specifically phosphorylates SC-35, resulting in p53 suppression and cyclin D1 upregulation. Elevated levels of SC-35 correlate with aggressive phenotype in several cancer types including lung squamous cell carcinoma and lung adenocarcinoma. SC-35 has oncogenic function in tumor progression of human papilloma virus (HPV)-infected cells.
外形
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
产品性质
| 质量水平 | 200 |
| 生物来源 | mouse |
| 抗体形式 | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| 克隆 | SC-35, monoclonal |
| 形式 | buffered aqueous solution |
| 分子量 | ~35 kDa |
| species reactivity | mouse, human, frog, rat, quail, hamster, porcine |
| 浓度 | ~1.0 mg/mL |
| technique(s) | ELISA: suitable dot blot: suitable immunoblotting: suitable immunofluorescence: 0.125-0.25 μg/mL using HeLa cells immunohistochemistry: suitable immunoprecipitation (IP): suitable |
| UniProt登记号 | Q01130 |
| 运输 | dry ice |
| 储存温度 | −20℃ |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | WGK 1 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
