产品说明
一般描述
SMAD6 is a member of inhibitory Smads (I-Smad) family. It consists of two conserved domains, the N-terminal MH1 and C-terminal MH2, that are separated by a proline-rich linker region of varying length.
免疫原
peptide corresponding to the N-terminal region of human SMAD6, conjugated to KLH. The corresponding sequence is identical in rat and differs by a single amino acid in mouse.
应用
Anti-SMAD6 (N-terminal) antibody produced in rabbit has been used in:
- immunoblotting
- immunoprecipitation
- immunofluorescence
生化/生理作用
SMAD6 plays an important role in transforming growth factor-β (TGF-β) pathway. It regulates cell proliferation, apoptosis and differentiation. SMAD6 is a key negative regulator of bone morphogenetic protein (BMP) signaling in the cytoplasm and in the nucleus. SMAD6 inhibits SMAD signaling in the nucleus by interacting with transcriptional repressors such as HOXC-8, class I histone deacetylases (HDACs) and transcriptional corepressor C-terminal binding protein (CtBp).
外形
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| NACRES | NA.41 |
产品性质
| 生物来源 | rabbit |
| 质量水平 | 200 |
| 偶联物 | unconjugated |
| 抗体形式 | affinity isolated antibody |
| antibody product type | primary antibodies |
| 克隆 | polyclonal |
| 形式 | buffered aqueous solution |
| 分子量 | antigen ~60 kDa |
| species reactivity | human, mouse |
| 浓度 | ~1.0 mg/mL |
| technique(s) | immunoprecipitation (IP): 2.5-5 μg using lysates of HEK-293T cells over-expressing human SMAD6 indirect immunofluorescence: 5-10 μg/mL using mouse 3T3 cells western blot: 1-2 μg/mL using whole extracts of HEK-293T cells over-expressing human SMAD6 |
| UniProt登记号 | O43541 |
| 运输 | dry ice |
| 储存温度 | −20℃ |
| Gene Information | human ... SMAD6(4091) rat ... Smad6(367100) |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
