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Anti-Shiga Toxin 1, B Subunit-FITC antibody, Mouse monoclonal

品牌
Sigma-Aldrich
货号
SAB4200799
规格纯度
clone 13C4, purified from hybridoma cell culture
参考价格
6333.77 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

Monoclonal Anti-Shiga Toxin 1, B Subunit-FITC (mouse IgG1 isotype) is derived from the hybridoma 13C4 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice. The Shiga toxins are a family of related protein toxins secreted by certain types of bacteria. Shiga toxin (Stx) is produced by Shigella dysenteriae; whereas, the Shiga-like toxins, Stx1 and Stx2, with a few known isoforms, are secreted by specific strains of Escherichia coli named Shiga-toxin-producing E. coli (STEC) such as E. coli O157:H7. Stx1 is identical to Stx and the Stx2 isoforms shares 60% similarity with STX. Howevere, the toxin structure is conserved. Shiga toxins consist of two polypeptides: A and B.

特异性

Monoclonal Anti-Shiga Toxin 1, B Subunit -FITC recognizes the B subunit of Shiga holotoxin.

免疫原

Purifed Shiga-like toxin from E. coli H30.

应用

Anti-Shiga Toxin 1, B Subunit-FITC antibody, Mouse monoclonal may be used in flow cytometry.

生化/生理作用

The STX catalytic A subunit has RNA N-glycosidase activity that inhibits eukaryotic protein synthesis. The B subunits form a pentamer, which recognizes and binds to the functional cell-surface receptor globotriaosylceramide [Gb3, Galα (1-4)-Galβ (1-4)-Glcβ1-ceramide]. Shiga toxins are implicated in bloody diarrhea and hemorrhagic colitis in humans, which may lead to fatal systemic complications. Gb3 is overexpressed in membranes of numerous tumor cells, therefore, STxB binding to Gb3 receptors may be useful in cancer cell imaging.

外形

Supplied as a solution in 0.01 M phosphate buffered saline pH 7.4 and 15 mM sodium azide as a preservative.

储存及稳定性

For continuous use, store at 2–8 ℃ protected from light for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours. Protect from prolonged exposure to light.

其他说明

In order to obtain best results in different techniques and preparations we recommend determining optimal working concentration by titration test.

免责声明

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

NACRESNA.41

产品性质

质量水平200
生物来源mouse
偶联物FITC conjugate
抗体形式purified from hybridoma cell culture
antibody product typeprimary antibodies
克隆13C4, monoclonal
描述Research area: Microbiome
形式buffered aqueous solution
species reactivityE. coli
储存条件protect from light
浓度~1 mg/mL
technique(s)flow cytometry: 2-4 μg/test using human RAMOS cells pretreated with recombinant Shiga toxin 1, B subunit
同位素/亚型IgG1
UniProt登记号Q7BQ98
运输dry ice
储存温度−20℃

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 3

Sigma-Aldrich

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