产品说明
一般描述
SDHA, also known as Succinate dehydrogenase flavoprotein-mitochondrial subunit, or Flavoprotein subunit of complex II (Fp), and encoded by the gene symbol SDHA/ SDH2/ SDHF, is a member of the FAD-dependent oxidoreductase 2 family and the FRD/SDH subfamily. Flavoprotein (FP) subunit of succinate dehydrogenase (SDH) is involved in the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q). Recent studies have indicated that SDHA may act as a tumor suppressor. SDHA is composed of four subunits: the flavoprotein (FP) SDHA, iron-sulfur protein (IP) SDHB, and a cytochrome b560 composed of SDHC and SDHD. SDHA interacts with SDHAF2/SDH5 in the process required for FAD attachment. Additionally, FDHA has been shown to interact with TRAP1. Mutations in the SDHA gene have been associated with Mitochondrial complex II deficiency (MT-C2D), a disorder of the mitochondrial respiratory chain with heterogeneous clinical manifestations. Additionally, mutations in the SDHA gene have been implied in Leigh syndrome or Kearns-Sayre syndrome, Cardiomyopathy dilated 1GG (CMD1GG), and Paragangliomas 5 (PGL5).
免疫原
GST-tagged recombinant protein corresponding to the C-terminus of Human SDHA.
Epitope: Immunogen is near the C-terminal.
应用
Anti-SDHA, clone 7H11.2 is an antibody against SDHA for use in WB, IH.
Research Category
Neuroscience
Research Sub Category
Neurodegenerative Diseases
Western Blotting Analysis: A 0.5 µg/mL dilution from a representative lot detected SDHA in 10 µg of C2C12 and HeLa cell lysates.
Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected SDHA in human cerebral cortex and human cardiac myocytes tissues.
质量
Evaluated by Western Blotting in Human brain tissue lysate.
Western Blotting Analysis: A 0.5 µg/mL diultion of this antibody detected SDHA in 10 µg of Human brain tissue lysate.
目标描述
~68 kDa observed
外形
Format: Purified
Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Protein G Purified
储存及稳定性
Stable for 1 year at 2-8°C from date of receipt.
其他说明
Concentration: Please refer to lot specific datasheet.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
eCl@ss | 32160702 |
产品性质
质量水平 | 100 |
生物来源 | mouse |
抗体形式 | purified immunoglobulin |
antibody product type | primary antibodies |
克隆 | 7H11.2, monoclonal |
species reactivity | mouse, human |
technique(s) | immunohistochemistry: suitable western blot: suitable |
同位素/亚型 | IgG2aκ |
NCBI登记号 | NP_004159 |
UniProt登记号 | P31040 |
运输 | wet ice |
Gene Information | human ... SDHA(6389) |
安全信息
储存分类代码 | 12 - Non Combustible Liquids |
WGK | WGK 1 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |