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Anti-Regnase-1/Zc3h12a Antibody, clone 15D11

品牌
Sigma-Aldrich
货号
MABF936
规格纯度
clone 15D11, from rat
参考价格
5520.38 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

Ribonuclease ZC3H12A (UniProt Q5D1E7; also known as MCP-induced protein 1, Regnase-1, Zinc finger CCCH domain-containing protein 12A) is encoded by the Zc3h12a (also known as Mcpip, Mcpip1) gene (Gene ID 230738) in murine species. Regnase-1 is an endonuclease that is involved in post-transcriptional gene regulation by cleaving target mRNAs or the terminal loop of pre-microRNAs. Regnase-1 negatively regulates Il6, Il12b, Il2, Icos, Ox40, Ctla4, cRel mRNAs, as well as its own mRNA (Zc3h12a). In T cells, regnase-1 and an RNA-binding protein roquin act together to inhibit T helper (TH) cell differentiation by negatively regulating mRNAs encoding the TH17 cell-promoting factors IL-6, ICOS, c-Rel, IRF4, IκBNS and IκBζ. This cooperation requires RNA-binding activity of roquin and the endonuclease activity of regnase-1. Upon T-cell receptor (TCR) activation following antigen binding, however, both roquin and regnase-1 are cleaved and inactivated by the paracaspase MALT1, allowing TH17 differentiation to proceed.

特异性

Clone 15D11 detected regnase-1 in wild-type, but not Zc3h12a-/-, mouse embryonic fibroblasts/MEFs (Jeltsch, K.M., et al. (2014). Nat. Immunol. 15(11):1079-1089).

免疫原

Recombinant full-length mouse regnase-1.

应用

Western Blotting Analysis: A representative lot detected regnase-1 in lysates from wild-type, but not Zc3h12a-/-, mouse embryonic fibroblasts/MEFs (Jeltsch, K.M., et al. (2014). Nat. Immunol. 15(11):1079-1089).
This Anti-Regnase-1/Zc3h12a Antibody, clone 15D11 is validated for use in Western Blotting for the detection of Regnase-1/Zc3h12a.
Research Sub Category
Immunoglobulins & Immunology
Research Category
Inflammation & Immunology

质量

Evaluated by Western Blotting in MEF1 cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected Regnase-1/Zc3h12a in 10 µg of MEF1 cell lysate.

目标描述

~70 kDa observed.

外形

Purified rat monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified
Protein G Purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss32160702
NACRESNA.41

产品性质

质量水平100
生物来源rat
抗体形式purified immunoglobulin
antibody product typeprimary antibodies
克隆15D11, monoclonal
species reactivitymouse
technique(s)western blot: suitable
同位素/亚型IgG2aκ
NCBI登记号NP_694799.1
UniProt登记号Q5D1E7
运输wet ice

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 2
闪点(F)Not applicable
闪点(C)Not applicable

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