产品说明
一般描述
RASSF1, also known as Ras association domain-containing protein 1, and encoded by the gene RASSF1/RDA32, is an important cell cycle and cell proliferation regulator protein. RASSF1 acts as a tumor suppressor and operates within the death receptor dependent apoptotic pathways. Additionally, RASSF1 inhibits proliferation at the G1/S boundary by regulating the accumulation of cyclin D1 protein. RASSF1 is localized to the cytoplasm and cytoskeleton depending upon cellular environment. RASSF1 localizes to cytoplasmic microtubules during interphase, to bipolar centrosomes associated with microtubules during prophase, to spindle fibers and spindle poles at metaphase and anaphase, to the midzone during early telophase, and to the midbody in late telophase in cells. The protein also co-localizes with MDM2 in the nucleus. RASSF1 is expressed in multiple isoforms but in generally it is ubiquitously expressed but down regulated in cancer and tumor cells.
免疫原
GST-tagged recombinant protein corresponding to human RASSF1.
应用
Detect RASSF1 using this Anti-RASSF1 Antibody, clone 5C9.1 validated for use in western blotting & IHC.
Immunohistochemistry Analysis: A 1:50-500 dilution from a representative lot detected RASSF1 in human kidney and human prostate cancer tissue.
质量
Evaluated by Western Blotting in human brain tissue lysate.
Western Blotting Analysis: 2.0 µg/mL of this antibody detected RASSF1 in 10 µg of human brain tissue lysate.
目标描述
~39 kDa observed
联系
Replaces: MABE526
外形
Protein G Purified
Format: Purified
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| eCl@ss | 32160702 |
| NACRES | NA.41 |
产品性质
| 质量水平 | 100 |
| 生物来源 | mouse |
| 抗体形式 | purified immunoglobulin |
| antibody product type | primary antibodies |
| 克隆 | 5C9.1, monoclonal |
| species reactivity | human |
| technique(s) | immunohistochemistry: suitable western blot: suitable |
| 同位素/亚型 | IgG1κ |
| NCBI登记号 | NP_009113 |
| UniProt登记号 | Q9NS23 |
| 运输 | wet ice |
| Gene Information | human ... RASSF1(11186) |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | WGK 1 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
