产品说明
一般描述
Puromycin is an aminonucleoside antibiotic, derived from the Streptomyces alboniger bacterium, that functions as a protein synthesis inhibitor that blocks translation through premature chain termination in the ribosome. Monoclonal antibodies to puromycin may be used with standard immunochemical methods to directly monitor translation, a method known as surface sensing of translation (SUnSET). Part of the molecule resembles the 3′ end of the aminoacylated tRNA, making it useful for protein translation analysis. Puromycin induces DNA fragmentation in thymocytes and in human HL-60 leukemia cells.
This product is a conjugated version of Cat. No. MABE343.
特异性
Demonstrated to react with Human test sample, preincubated with Puromycin. Predicted to react with all species when test sample is incubated with Puromycin.
This antibody detects Puromycin-incorporated neosynthesized proteins.
免疫原
Puromycin-incorporated neosynthesized proteins at multiple molecular weights
应用
Research Sub Category
Cell Cycle, DNA Replication & Repair
Research Category
Epigenetics & Nuclear Function
This Anti-Puromycin, clone 12D10 is validated for use in Immunocytochemistry for the detection of Puromycin.
质量
Evaluated by Immunocytochemistry in untreated and Puromycin treated HeLa cells.
Immunocytochemistry Analysis: A 1:2,500 dilution of this antibody detected Puromycin-incorporated neosynthesized proteins in HeLa cells treated with Puromycin only.
Alexa Fluor® is a registered trademark of Life Technologies
目标描述
Refer to Cat. No. MABE343 for observed molecular weight information.
外形
Purified rat monoclonal conjugate in buffer containing PBS and 15 mg/ml BSA with 0.1% Sodium Azide.
Protein G Purified
储存及稳定性
Stable for 1 year at 2-8°C from date of receipt.
法律信息
ALEXA FLUOR is a registered trademark of Life Technologies
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| eCl@ss | 32160702 |
| NACRES | NA.41 |
产品性质
| 质量水平 | 100 |
| 生物来源 | mouse |
| 偶联物 | ALEXA FLUOR® 488 |
| 抗体形式 | purified antibody |
| antibody product type | primary antibodies |
| 克隆 | 12D10, monoclonal |
| species reactivity | human |
| 浓度 | 0.5 mg/mL |
| technique(s) | immunocytochemistry: suitable |
| 运输 | wet ice |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | WGK 2 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
