产品说明
一般描述
Puromycin is an aminonucleoside antibiotic, derived from the Streptomyces alboniger bacterium, that functions as a protein synthesis inhibitor that blocks translation through premature chain termination in the ribosome. Monoclonal antibodies to puromycin may be used with standard immunochemical methods to directly monitor translation, a method known as surface sensing of translation (SUnSET). Part of the molecule resembles the 3′ end of the aminoacylated tRNA, making it useful for protein translation analysis. Puromycin induces DNA fragmentation in thymocytes and in human HL-60 leukemia cells.
特异性
Demonstrated to react with Human test sample, preincubated with Puromycin. Predicted to react with all species when test sample is incubated with Puromycin.
应用
Immunocytochemistry Analysis: A 1:2,000 dilution from a representative lot detected Puromycin-incorporated neosynthesized proteins in HeLa cells treated with Puromycin. However, a decrease in signal is observed with the addition of Cycloheximide, an inhibitor of protein biosynthesis in eukaryotic organisms.
Western Blotting Analysis: A representative lot from an independent laboratory detected Puromycin-incorporated neosynthesized proteins in WB (David, A., et al. (2012). J Cell Biol. 197(1):45-57.; Schmidt, E., K., et al. (2009). Nat Methods. 6(4):275-277.).
Immunofluorescence Analysis: A representative lot from an independent laboratory detected Puromycin-incorporated neosynthesized proteins in IF (David, A., et al. (2012). J Cell Biol. 197(1):45-57.; Schmidt, E., K., et al. (2009). Nat Methods. 6(4):275-277.).
Fluorescence Activated Cell Sorting Analysis: A representative lot from an independent laboratory detected Puromycin-incorporated neosynthesized proteins in FACS (David, A., et al. (2012). J Cell Biol. 197(1):45-57.; Schmidt, E., K., et al. (2009). Nat Methods. 6(4):275-277.).
Alexa Fluor® is a registered trademark of Life Technologies
Anti-Puromycin antibody, clone 4G11 detects puromycin incorporated into protein. Monoclonal antibodies to puromycin may also be used with standard immunochemical methods.
质量
Evaluated by Western Blotting in HEK293 cell lysates treated with Puromycin and Cyclohexamide, or with Puromycin only.
Western Blotting Analysis: A 1:12,500 dilution of this antibody detected Puromycin-incorporated neosynthesized proteins in HEK293 cell lysates treated with Puromycin only. This antibody also detected small mounts of puromycin in HEK293 cells treated with Puromycin and Cyclohexamide.
目标描述
Puromycin is incorporated in neosynthesized proteins. In the presense of Puromycin only, this antibody detects Puromycin-incorporated neosynthesized proteins at multiple molecular weights. However, a decrease in signal is observed in the co-presense of Cycloheximide, an inhibitor of protein biosynthesis in eukaryotic organisms.
外形
Protein G Purified
Format: Purified
法律信息
ALEXA FLUOR is a registered trademark of Life Technologies
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
产品性质
质量水平 | 100 |
生物来源 | mouse |
抗体形式 | purified immunoglobulin |
antibody product type | primary antibodies |
克隆 | 4G11, monoclonal |
species reactivity | human |
species reactivity (predicted by homology) | all |
technique(s) | flow cytometry: suitable immunocytochemistry: suitable immunofluorescence: suitable western blot: suitable |
同位素/亚型 | IgG1κ |
运输 | wet ice |
安全信息
储存分类代码 | 12 - Non Combustible Liquids |
WGK | WGK 1 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |