产品说明
一般描述
PTPRA is a transmembrane phosphatase that regulates cell proliferation, cell adhesion, integrin signaling, and growth factor response. This phosphatase also activates proteins of the Src family. PTPRA inhibits cell growth in breast cancer and contributes to tumorigenesis in colon carcinoma . Anti-PTPRA antibody is specific for human and mouse PTPRA. In immunoblotting, detection of the PTPRA band is specifically inhibited by the immunizing peptide.
Protein tyrosine phosphatase alpha (PTPRA) is a transmembrane phosphatase with a relatively short, highly glycosylated extracellular domain, a transmembrane domain, and a tandem phosphatase domain. The amino-terminal domain predominantly mediates catalytic activity, whereas the carboxyterminal domain serves as a regulatory subunit.
免疫原
synthetic peptide corresponding to an internal region of human PTPRA, conjugated to KLH. The corresponding sequence is identical in mouse and differs by one amino acid in rat.
应用
Anti-PTPRA antibody produced in rabbit has been used in immunoblotting and immunofluorescence.
Anti-PTPRA antibody is suitable for use in western blot (0.25-0.5 μg/mL using lysates of NIH-3T3 or PC12 cells) and indirect immunofluorescence (5-10 μg/mL using paraformaldehyde fixed HUVEC cells).
生化/生理作用
Protein tyrosine phosphatase alpha (PTPRA) is implicated in several signaling pathways, including insulin receptor signaling, cellular transformation and cell spreading and migration. It regulates cell proliferation, cell adhesion, integrin signaling, and growth factor response. PTPRA inhibits cell growth in breast cancer and contributes to tumorigenesis in colon carcinoma.
外形
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
产品性质
| 生物来源 | rabbit |
| 质量水平 | 200 |
| 偶联物 | unconjugated |
| 抗体形式 | affinity isolated antibody |
| antibody product type | primary antibodies |
| 克隆 | polyclonal |
| 形式 | buffered aqueous solution |
| 分子量 | antigen ~90 kDa |
| species reactivity | mouse, human |
| 浓度 | ~1.0 mg/mL |
| technique(s) | indirect immunofluorescence: 5-10 μg/mL using paraformaldehyde fixed HUVEC cells. western blot: 0.25-0.5 μg/mL using lysates of NIH-3T3 or PC12 cells. |
| UniProt登记号 | P18433 |
| 运输 | dry ice |
| 储存温度 | −20℃ |
| Gene Information | human ... PTPRA(5786) mouse ... PTPRA(19262) |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
