位置:首页 > 品牌 > Sigma-Aldrich >

Anti-PTPN22 Antibody, clone 3B3.1

品牌
Sigma-Aldrich
货号
MABS480
规格纯度
culture supernatant, clone 3B3.1, from mouse
参考价格
6190.47 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

Tyrosine-protein phosphatase non-receptor type 22 (EC:3.1.3.48; UniProt Q9Y2R2; also known as Hematopoietic cell protein-tyrosine phosphatase 70Z-PEP, Lymphoid phosphatase, Lymphoid-specific protein tyrosine phosphatase, Lyp, PEP, PEST-domain phosphatase, Protein tyrosine phosphatase non-receptor type 8) is encoded by the PTPN22 (also known as LYP, LYP1, LYP2, PTPN8) gene (Gene ID 26191) in human. PTPN22/Lyp is expressed in all hematopoietic cells and belongs to the PEST group of nonreceptor classical class I protein tyrosine phosphatases (PTPs). Lyp is a negative regulator of TCR signal transduction by targeting phosphorylated Lck (Y394), Fyn (Y427), and Zap-70, as well as phospho-sites on TCRζ, CD3ε, Vav, and Vcp. Lyp utilizes its proline-rich region 1 to interact with the SH3 domain of C-terminal Src kinase (Csk), resulting in enhanced inhibitory tuning of Src family kinases and TCR signal transduction. TRAF3 is also shown to bind the proline-rich region 1 of Lyp in myeloid cells and impact its turnover. Additional binding partners of Lyp include the adaptor molecule Grb2, the E3 ligase c-Cbl.

特异性

Clone 3B3.1 is expected to react with spliced isoform 1 (LyP1), isoform 3, and isoform 4 (LyP3), but not isoform 2 (LyP2) or isoform 5. Reactivity toward isoform 6 (PTPN22.6) has not been tested.

免疫原

Epitope: C-terminal
GST-tagged recombinant protein corresponding to the C-terminal of human PTPN22.

应用

This Anti-PTPN22 Antibody, clone 3B3.1 is validated for use in Western Blotting for the detection of PTPN22.
Research Category
Signaling
Research Sub Category
Developmental Signaling

质量

Evaluated by Western Blotting in human burkitt′s lymphoma Ramos cell lysate.

Western Blotting Analysis: A 1:2,000 dilution of this antibody detected PTPN22 in 10 µg of human burkitt′s lymphoma Ramos cell lysate.

目标描述

~92 kDa observed. 91.7 kDa (isoform 1; Lyp1), 85.1 kDa (isoform 3), 88.6 kDa (isoform 4; Lyp3) calculated. Uncharacterized band(s) may appear in some lysates.

外形

Unpurified
Mouse monoclonal IgG1κ in supernatant without preservatives.

储存及稳定性

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss 32160702

产品性质

质量水平100
生物来源mouse
抗体形式culture supernatant
antibody product typeprimary antibodies
克隆3B3.1, monoclonal
species reactivityhuman
technique(s)western blot: suitable
同位素/亚型IgG1κ
NCBI登记号NP_057051
UniProt登记号Q9Y2R2
运输dry ice

安全信息

储存分类代码10 - Combustible liquids
WGKWGK 2

Sigma-Aldrich

推荐产品
| 首页 | 联系我们 | 会员服务 | 广告服务 | 友情链接 |
版权所有 CopyRight © 2008-2024 粤ICP备08119708号