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Anti-PrP, clone DRM2-118 Antibody

品牌
Sigma-Aldrich
货号
MABN768
规格纯度
clone DRM2-118, from mouse
参考价格
5520.38 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

The protein named PrP is the cellular gene for the prion protein. The normal form appears to play a role in neuronal development and synaptic plasticity, iron transport and myelin sheath maintenance. PrP has both membrane associated and cytoplasmic/nuclear isoforms. The primary isoform is localized to the cellular membrane in lipid anchor clusters, as well as in the Golgi. Isoform 2 is cytoplasmic that becomes localized to the nucleus. Prp is normally a monomeric protein but a disease associated mutated form will form protease resistant oligomers often with another protein beta amyloid. The mutated form is found in high quantity in the brain of humans and animals infected with the degenerative neurological diseases such as Kuru, Creutzfeldt-Jakob disease (CJD), Gerstmann-Straussler syndrome (GSS), Scrapie, and Bovine Spongiform Encephalopathy (BSE) and Transmissible Mink Encephalopathy.

特异性

DRM1-60 showed strong binding to only one peptide (#29: NQVYYRPVDQYS) suggesting a linear epitope corresponding to SHaPrP(159–170), located in b2-a2 loop. This epitope assignment is consistent with the binding of DRM1-60 to PrPC to all species tested, except human and horse, which both harbor substitutions in this region. While antibodies with epitopes similar to DRM2-118 have been described,(36) DRM1-60 and DRM1-31 have unique epitopes not previously reported. DRM1-60 appears to bind a linear epitope localized to the b2-a2 loop, while DRM1-31 has a conformational epitope located on the exposed surface formed by the close proximity of the b2-a2- helix loop, the N-terminus of the a2 helix, and the C-terminus of the a3 helix.

免疫原

Recombinant protein corresponding to syrian hamster PrP.

应用

Anti-PrP, clone DRM2-118 | MABN768 is an antibody against PrP for use in Western Blotting, Immunohistochemistry, ELISA.
Research Category
Neuroscience
Western Blotting Analysis: 0.5 µg/mL from a representative lot detected PrP in 10 µg of rat brain tissue lysate.
ELISA Analysis: A representative lot detected PrP in recombinant PrP proteins from multiple species by ELISA using DRM1-31, DRM1-60, and DRM2-118 (Stanker, L.H., et al. (2012). 31(5):314-24).
Immunohistochemistry Analysis: A representative lot detected PrP in hamster brain sections tissue with DRM1-31, DRM1-60, and DRM2-118 (Stanker, L.H., et al. (2012). 31(5):314-24).
Western Blotting Analysis: A representative lot detected PrP in normal brain extract from multiple spcecies using DRM2-118. DRM1-60, and DRM1-31 lysate (Stanker, L.H., et al. (2012). 31(5):314-24).
Research Sub Category
Developmental Signaling

质量

Evaluated by Western Blotting in mouse brain tissue lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected PrP in 10 µg of mouse brain tissue lysate.

目标描述

~36 kDa observed. Uncharacterized band(s) may appear in some lysates.

外形

Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified
Protein G Purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss32160702
NACRESNA.41

产品性质

质量水平100
生物来源mouse
抗体形式purified immunoglobulin
antibody product typeprimary antibodies
克隆DRM2-118, monoclonal
species reactivitybovine, porcine, rat, hamster, mouse, sheep, human
technique(s)ELISA: suitable
immunohistochemistry: suitable
western blot: suitable
同位素/亚型IgG2bκ
NCBI登记号XP_005068717
UniProt登记号P04273
运输wet ice
Gene Informationhuman ... PRNP(5621)

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 1
闪点(F)Not applicable
闪点(C)Not applicable

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