产品说明
一般描述
Anti-Protein kinase C antibody, Mouse monoclonal (mouse IgG2a isotype) is derived from the MC5 hybridoma produced by the fusion of X-63 myeloma cells and splenocytes from a BALB/c mouse immunized with purified bovine brain protein kinase C1. Protein kinase C (PKC), is a family of phospholipid- and calcium-activated, diacylglycerol (DAG)-dependent serine/threonine kinases.
特异性
Monoclonal Anti-Protein kinase C antibody recognizes an epitope located within the amino acid residues 296- 317, at the hinge region, close to or at the trypsin cleavage site of Protein kinase C molecule . The antibody specifically recognizes Protein kinase C from human, rat, bovine, mouse, chicken, rabbit and fish origin.
应用
Anti-Protein kinase C antibody has been used in:
- immunoblotting
- immunohistochemistry
- immunofluorescence
- immunoprecipitation
生化/生理作用
Protein kinase C (PKC) play a central role in a variety of cell processes, including: intracellular signal transduction, cell proliferation, apoptosis, differentiation, migration and adhesion, tumor promotion, cardiac hypertrophy, angiogenesis, platelet function, inflammation and oncogenesis. Neuronal PKC modulates several functions such as nerve cell excitability, neuronal plasticity and release of neurotransmitters. PKC activity is regulated by two mechanisms- firstly by phosphorylation which regulates the active site and subcellular localization of the enzyme, secondly by secondary messengers which facilitate association of PKC on membrane. The PKC activity is strongly associated with tumor initiation and progression and have been implemented as a target for treatment of cancer progression and diabetic retinopathy.
外形
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Purified bovine brain protein kinase C
储存及稳定性
Store at –20℃. For continuous use, store at 2–8℃ for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
免责声明
Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| NACRES | NA.44 |
产品性质
| 质量水平 | 200 |
| 生物来源 | mouse |
| 抗体形式 | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| 克隆 | MC5, monoclonal |
| 形式 | buffered aqueous solution |
| 分子量 | antigen ~80 kDa |
| species reactivity | rat, fish, bovine, rabbit, mouse, human, chicken |
| 浓度 | ~1.0 mg/mL |
| technique(s) | immunoblotting: 1.25-2.5 μg/mL using human bone marrow neuroblast SH-SY5Y cell extract immunocytochemistry: suitable immunofluorescence: suitable immunoprecipitation (IP): suitable |
| 同位素/亚型 | IgG2a |
| UniProt登记号 | P04409 |
| 运输 | dry ice |
| 储存温度 | −20℃ |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
