产品说明
一般描述
Monoclonal Anti-Profilin 1 (mouse IgG1 isotype) is derived from the hybridoma Profilin1-3 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to a sequence located near the C-terminus of human profiling 1, conjugated to keyhole limpet hemocyanin (KLH). Profilin-1 (Pfn1), a major actin-regulatory protein is mapped to human chromosome17p13. It is the most ubiquitous and abundant protein and is highly expressed throughout development and adulthood in most tissues including brain.
免疫原
synthetic peptide corresponding to a sequence located near the C-terminus of human profilin 1, conjugated to KLH. The corresponding sequence is identical in mouse and rat profilin 1.
应用
Monoclonal Anti-Profilin 1 antibody produced in mouse has been used in immunoblotting and immunoprecipitation.
生化/生理作用
Profilin-1 (Pfn1) is involved in actin polymerization in response to extracellular signals. Profilins were shown to be important for normal cell proliferation, differentiation and motility. Profilin 1 is a potent regulator of actin filament dynamics. It is suggested to act as a tumor suppressor protein based on its reduced expression in several types of invasive cancers and its ability to suppress tumorigenicity when over expressed in breast cancer cells. Deletion of the profilin 1 gene leads to an embryonic lethal phenotype.
外形
0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| NACRES | NA.41 |
产品性质
| 质量水平 | 200 |
| 生物来源 | mouse |
| 偶联物 | unconjugated |
| 抗体形式 | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| 克隆 | Profilin 1-3, monoclonal |
| 形式 | buffered aqueous solution |
| 分子量 | antigen ~15 kDa |
| species reactivity | canine, bovine, human, rat, mouse |
| 浓度 | ~1.0 mg/mL |
| technique(s) | immunoprecipitation (IP): suitable western blot: 1-2 μg/mL using whole extracts of HeLa or NRK cells |
| 同位素/亚型 | IgG1 |
| UniProt登记号 | P07737 |
| 运输 | dry ice |
| 储存温度 | −20℃ |
| Gene Information | human ... PFN1(5216) mouse ... Pfn1(18643) rat ... Pfn1(64303) |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
