产品说明
一般描述
Protein phosphatase 2A (PP2A) is a family of ubiquitous heterotrimeric proteins that catalyze the removal of phosphate groups from serine and threonine residues. The PP2A enzymes contain core structural (A) and catalytic (C) subunits and variable regulatory B subunits. The B subunits regulate their catalytic activity, as well as dictate substrate specificity and subcellular localization. Recent studies have suggested that B56 epsilon subunits regulate p53-mediated apoptotic pathways, and can induce or suppress apoptosis depending on the cellular context. PP2A enzymes are also regulated by posttranslational modifications and endogenous inhibitors.
特异性
This antibody is specific for the B56 regulatory subunit of Protein phosphatase 2A (PP2A).
免疫原
His-tagged recombinant protein corresponding to human PP2A reg. subunit B56 epsilon (PPP2R5E).
应用
Research Category
Neuroscience
Apoptosis & Cancer
This Anti-PP2A reg. subunit B56 epsilon (PPP2R5E) Antibody, clone 5A5-1F3 is validated for use in Western Blotting, ICC, Immunoprecipitation for the detection of PP2A reg. subunit B56 epsilon (PPP2R5E).
Western Blot Analysis: A 1:1,000 dilution from a representative lot detected PP2A reg. subunit B56 epsilon (PPP2R5E) in 10 µg of U2OS cell lysate and mouse brain tissue lysate.
Immunocytochemistry Analysis: A representative lot detected PP2A reg. subunit B56 epsilon (PPP2R5E) in U2OS cells. (Max F. Perutz Laboratories, Medical University of Vienna, Austria).
Immunoprecipitation Analysis: A representative lot immunoprecipitated endogenous PP2A reg. subunit B56 epsilon (PPP2R5E) in a panel of U2OS cell lysates expressing the B56 alpha, beta, gamma, delta, and epsilon regulatory subunit of PP2A (Max F. Perutz Laboratories, Medical University of Vienna, Austria).
Research Sub Category
Kinases & Phosphatases
Apoptosis - Additional
质量
Evaluted by Western Blot in NIH/3T3 cell lysate.
Western Blot Analysis: A 1:1,000 dilution from a representative lot detected PP2A reg. subunit B56 epsilon (PPP2R5E) in 10 µg of NIH/3T3 cell lysate.
目标描述
~50 kDa observed
外形
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified
Protein G Purified
储存及稳定性
Stable for 1 year at 2-8°C from date of receipt.
分析说明
Control
NIH/3T3 cell lysate
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
产品性质
质量水平 | 100 |
生物来源 | mouse |
抗体形式 | purified immunoglobulin |
antibody product type | primary antibodies |
克隆 | 5A5-1F3, monoclonal |
species reactivity | human, mouse |
technique(s) | immunocytochemistry: suitable immunoprecipitation (IP): suitable western blot: suitable |
同位素/亚型 | IgG1κ |
NCBI登记号 | NP_006237 |
UniProt登记号 | Q16537 |
运输 | wet ice |
安全信息
储存分类代码 | 12 - Non Combustible Liquids |
WGK | WGK 1 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |